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Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States

Dexamethasone (DEXA) is a potent immunosupressant and anti-inflammatory agent whose main side effects are muscle atrophy and insulin resistance in skeletal muscles. In this context, leucine supplementation may represent a way to limit the DEXA side effects. In this study, we have investigated the ef...

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Autores principales: Zanchi, Nelo Eidy, Guimarães-Ferreira, Lucas, de Siqueira-Filho, Mário Alves, Felitti, Vitor, Nicastro, Humberto, Bueno, Carlos, Lira, Fábio Santos, Naimo, Marshall Alan, Campos-Ferraz, Patrícia, Nunes, Maria Tereza, Seelaender, Marília, de Oliveira Carvalho, Carla Roberta, Blachier, François, Lancha, Antonio Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546611/
https://www.ncbi.nlm.nih.gov/pubmed/23363994
http://dx.doi.org/10.3390/nu4121851
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author Zanchi, Nelo Eidy
Guimarães-Ferreira, Lucas
de Siqueira-Filho, Mário Alves
Felitti, Vitor
Nicastro, Humberto
Bueno, Carlos
Lira, Fábio Santos
Naimo, Marshall Alan
Campos-Ferraz, Patrícia
Nunes, Maria Tereza
Seelaender, Marília
de Oliveira Carvalho, Carla Roberta
Blachier, François
Lancha, Antonio Herbert
author_facet Zanchi, Nelo Eidy
Guimarães-Ferreira, Lucas
de Siqueira-Filho, Mário Alves
Felitti, Vitor
Nicastro, Humberto
Bueno, Carlos
Lira, Fábio Santos
Naimo, Marshall Alan
Campos-Ferraz, Patrícia
Nunes, Maria Tereza
Seelaender, Marília
de Oliveira Carvalho, Carla Roberta
Blachier, François
Lancha, Antonio Herbert
author_sort Zanchi, Nelo Eidy
collection PubMed
description Dexamethasone (DEXA) is a potent immunosupressant and anti-inflammatory agent whose main side effects are muscle atrophy and insulin resistance in skeletal muscles. In this context, leucine supplementation may represent a way to limit the DEXA side effects. In this study, we have investigated the effects of a low and a high dose of leucine supplementation (via a bolus) on glucose homeostasis, muscle mass and muscle strength in energy-restricted and DEXA-treated rats. Since the leucine response may also be linked to the administration of this amino acid, we performed a second set of experiments with leucine given in bolus (via gavage) versus leucine given via drinking water. Leucine supplementation was found to produce positive effects (e.g., reduced insulin levels) only when administrated in low dosage, both via the bolus or via drinking water. However, under DEXA treatment, leucine administration was found to significantly influence this response, since leucine supplementation via drinking water clearly induced a diabetic state, whereas the same effect was not observed when supplied via the gavage.
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spelling pubmed-35466112013-01-23 Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States Zanchi, Nelo Eidy Guimarães-Ferreira, Lucas de Siqueira-Filho, Mário Alves Felitti, Vitor Nicastro, Humberto Bueno, Carlos Lira, Fábio Santos Naimo, Marshall Alan Campos-Ferraz, Patrícia Nunes, Maria Tereza Seelaender, Marília de Oliveira Carvalho, Carla Roberta Blachier, François Lancha, Antonio Herbert Nutrients Article Dexamethasone (DEXA) is a potent immunosupressant and anti-inflammatory agent whose main side effects are muscle atrophy and insulin resistance in skeletal muscles. In this context, leucine supplementation may represent a way to limit the DEXA side effects. In this study, we have investigated the effects of a low and a high dose of leucine supplementation (via a bolus) on glucose homeostasis, muscle mass and muscle strength in energy-restricted and DEXA-treated rats. Since the leucine response may also be linked to the administration of this amino acid, we performed a second set of experiments with leucine given in bolus (via gavage) versus leucine given via drinking water. Leucine supplementation was found to produce positive effects (e.g., reduced insulin levels) only when administrated in low dosage, both via the bolus or via drinking water. However, under DEXA treatment, leucine administration was found to significantly influence this response, since leucine supplementation via drinking water clearly induced a diabetic state, whereas the same effect was not observed when supplied via the gavage. MDPI 2012-11-27 /pmc/articles/PMC3546611/ /pubmed/23363994 http://dx.doi.org/10.3390/nu4121851 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zanchi, Nelo Eidy
Guimarães-Ferreira, Lucas
de Siqueira-Filho, Mário Alves
Felitti, Vitor
Nicastro, Humberto
Bueno, Carlos
Lira, Fábio Santos
Naimo, Marshall Alan
Campos-Ferraz, Patrícia
Nunes, Maria Tereza
Seelaender, Marília
de Oliveira Carvalho, Carla Roberta
Blachier, François
Lancha, Antonio Herbert
Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title_full Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title_fullStr Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title_full_unstemmed Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title_short Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States
title_sort dose and latency effects of leucine supplementation in modulating glucose homeostasis: opposite effects in healthy and glucocorticoid-induced insulin-resistance states
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546611/
https://www.ncbi.nlm.nih.gov/pubmed/23363994
http://dx.doi.org/10.3390/nu4121851
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