Cargando…
Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs
A comprehensive proteome map of T-lymphoblastic leukemia cells and its alterations after daunorubicin, doxorubicin and mitoxantrone treatments was monitored and evaluated either by paired comparison with relevant untreated control and using multivariate classification of treated and untreated sample...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546648/ https://www.ncbi.nlm.nih.gov/pubmed/23443080 http://dx.doi.org/10.3390/ijms131215536 |
_version_ | 1782256079925673984 |
---|---|
author | Tyleckova, Jirina Hrabakova, Rita Mairychova, Katerina Halada, Petr Radova, Lenka Dzubak, Petr Hajduch, Marian Gadher, Suresh J. Kovarova, Hana |
author_facet | Tyleckova, Jirina Hrabakova, Rita Mairychova, Katerina Halada, Petr Radova, Lenka Dzubak, Petr Hajduch, Marian Gadher, Suresh J. Kovarova, Hana |
author_sort | Tyleckova, Jirina |
collection | PubMed |
description | A comprehensive proteome map of T-lymphoblastic leukemia cells and its alterations after daunorubicin, doxorubicin and mitoxantrone treatments was monitored and evaluated either by paired comparison with relevant untreated control and using multivariate classification of treated and untreated samples. With the main focus on early time intervals when the influence of apoptosis is minimized, we found significantly different levels of proteins, which corresponded to 1%–2% of the total amount of protein spots detected. According to Gene Ontology classification of biological processes, the highest representation of identified proteins for all three drugs belong to metabolic processes of proteins and nucleic acids and cellular processes, mainly cytoskeleton organisation and ubiquitin-proteasome pathway. Importantly, we observed significant proportion of changes in proteins involved in the generation of precursor metabolites and energy typical for daunorubicin, transport proteins participating in response to doxorubicin and a group of proteins of immune system characterising response to mitoxantrone. Both a paired comparison and the multivariate evaluation of quantitative data revealed daunorubicin as a distinct member of the group of anthracycline/anthracenedione drugs. A combination of identified drug specific protein changes, which may help to explain anti-cancer activity, together with the benefit of blocking activation of adaptive cancer pathways, presents important approaches to improving treatment outcomes in cancer. |
format | Online Article Text |
id | pubmed-3546648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-35466482013-01-23 Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs Tyleckova, Jirina Hrabakova, Rita Mairychova, Katerina Halada, Petr Radova, Lenka Dzubak, Petr Hajduch, Marian Gadher, Suresh J. Kovarova, Hana Int J Mol Sci Article A comprehensive proteome map of T-lymphoblastic leukemia cells and its alterations after daunorubicin, doxorubicin and mitoxantrone treatments was monitored and evaluated either by paired comparison with relevant untreated control and using multivariate classification of treated and untreated samples. With the main focus on early time intervals when the influence of apoptosis is minimized, we found significantly different levels of proteins, which corresponded to 1%–2% of the total amount of protein spots detected. According to Gene Ontology classification of biological processes, the highest representation of identified proteins for all three drugs belong to metabolic processes of proteins and nucleic acids and cellular processes, mainly cytoskeleton organisation and ubiquitin-proteasome pathway. Importantly, we observed significant proportion of changes in proteins involved in the generation of precursor metabolites and energy typical for daunorubicin, transport proteins participating in response to doxorubicin and a group of proteins of immune system characterising response to mitoxantrone. Both a paired comparison and the multivariate evaluation of quantitative data revealed daunorubicin as a distinct member of the group of anthracycline/anthracenedione drugs. A combination of identified drug specific protein changes, which may help to explain anti-cancer activity, together with the benefit of blocking activation of adaptive cancer pathways, presents important approaches to improving treatment outcomes in cancer. Molecular Diversity Preservation International (MDPI) 2012-11-22 /pmc/articles/PMC3546648/ /pubmed/23443080 http://dx.doi.org/10.3390/ijms131215536 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Tyleckova, Jirina Hrabakova, Rita Mairychova, Katerina Halada, Petr Radova, Lenka Dzubak, Petr Hajduch, Marian Gadher, Suresh J. Kovarova, Hana Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title | Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title_full | Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title_fullStr | Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title_full_unstemmed | Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title_short | Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs |
title_sort | cancer cell response to anthracyclines effects: mysteries of the hidden proteins associated with these drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546648/ https://www.ncbi.nlm.nih.gov/pubmed/23443080 http://dx.doi.org/10.3390/ijms131215536 |
work_keys_str_mv | AT tyleckovajirina cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT hrabakovarita cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT mairychovakaterina cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT haladapetr cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT radovalenka cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT dzubakpetr cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT hajduchmarian cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT gadhersureshj cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs AT kovarovahana cancercellresponsetoanthracyclineseffectsmysteriesofthehiddenproteinsassociatedwiththesedrugs |