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Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls

Exposure to persistent environmental pollutants, such as polychlorinated biphenyls (PCBs), is a risk factor for the development of congenital heart defects. MicroRNAs (miRNAs) have been shown to be involved in cardiac development. The objective of this study was to investigate changes in miRNA expre...

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Autores principales: Zhu, Chun, Yu, Zhang-Bin, Zhu, Jin-Gai, Hu, Xiao-Shan, Chen, Yu-Lin, Qiu, Yu-Fang, Xu, Zheng-Feng, Qian, Lin-Mei, Han, Shu-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546672/
https://www.ncbi.nlm.nih.gov/pubmed/23443104
http://dx.doi.org/10.3390/ijms131215955
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author Zhu, Chun
Yu, Zhang-Bin
Zhu, Jin-Gai
Hu, Xiao-Shan
Chen, Yu-Lin
Qiu, Yu-Fang
Xu, Zheng-Feng
Qian, Lin-Mei
Han, Shu-Ping
author_facet Zhu, Chun
Yu, Zhang-Bin
Zhu, Jin-Gai
Hu, Xiao-Shan
Chen, Yu-Lin
Qiu, Yu-Fang
Xu, Zheng-Feng
Qian, Lin-Mei
Han, Shu-Ping
author_sort Zhu, Chun
collection PubMed
description Exposure to persistent environmental pollutants, such as polychlorinated biphenyls (PCBs), is a risk factor for the development of congenital heart defects. MicroRNAs (miRNAs) have been shown to be involved in cardiac development. The objective of this study was to investigate changes in miRNA expression profiles during the differentiation of cardiomyocytes exposed to PCBs. For that purpose, PCBs (Aroclor 1254) at a concentration of 2.5 μmol/L were added on day 0 of differentiation of P19 mouse embryonal carcinoma cells into cardiac myocytes. The relative expression of miRNA genes was determined by miRNA microarray and real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) analyses. The microarray results revealed that 45 miRNAs, of which 14 were upregulated and 31 were downregulated, were differentially expressed in P19 cells treated with PCBs compared with control cells. The miRNA expression data was validated with real-time RT-PCR. The expression of certain potential target genes (Wnt1) was found to be reduced in P19 cells treated with PCBs, whereas the expression of other potential predicted target genes (GSK3β) was increased. Our results demonstrate a critical role of miRNAs in mediating the effect of PCBs during the differentiation of P19 cells into cardiac myocytes.
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spelling pubmed-35466722013-01-23 Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls Zhu, Chun Yu, Zhang-Bin Zhu, Jin-Gai Hu, Xiao-Shan Chen, Yu-Lin Qiu, Yu-Fang Xu, Zheng-Feng Qian, Lin-Mei Han, Shu-Ping Int J Mol Sci Article Exposure to persistent environmental pollutants, such as polychlorinated biphenyls (PCBs), is a risk factor for the development of congenital heart defects. MicroRNAs (miRNAs) have been shown to be involved in cardiac development. The objective of this study was to investigate changes in miRNA expression profiles during the differentiation of cardiomyocytes exposed to PCBs. For that purpose, PCBs (Aroclor 1254) at a concentration of 2.5 μmol/L were added on day 0 of differentiation of P19 mouse embryonal carcinoma cells into cardiac myocytes. The relative expression of miRNA genes was determined by miRNA microarray and real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) analyses. The microarray results revealed that 45 miRNAs, of which 14 were upregulated and 31 were downregulated, were differentially expressed in P19 cells treated with PCBs compared with control cells. The miRNA expression data was validated with real-time RT-PCR. The expression of certain potential target genes (Wnt1) was found to be reduced in P19 cells treated with PCBs, whereas the expression of other potential predicted target genes (GSK3β) was increased. Our results demonstrate a critical role of miRNAs in mediating the effect of PCBs during the differentiation of P19 cells into cardiac myocytes. Molecular Diversity Preservation International (MDPI) 2012-11-28 /pmc/articles/PMC3546672/ /pubmed/23443104 http://dx.doi.org/10.3390/ijms131215955 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhu, Chun
Yu, Zhang-Bin
Zhu, Jin-Gai
Hu, Xiao-Shan
Chen, Yu-Lin
Qiu, Yu-Fang
Xu, Zheng-Feng
Qian, Lin-Mei
Han, Shu-Ping
Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title_full Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title_fullStr Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title_full_unstemmed Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title_short Differential Expression Profile of MicroRNAs during Differentiation of Cardiomyocytes Exposed to Polychlorinated Biphenyls
title_sort differential expression profile of micrornas during differentiation of cardiomyocytes exposed to polychlorinated biphenyls
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546672/
https://www.ncbi.nlm.nih.gov/pubmed/23443104
http://dx.doi.org/10.3390/ijms131215955
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