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Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus

Japanese encephalitis (JE), a mosquito-borne viral disease, is endemic to the entire east and southeast Asia, and some other parts of the world. Currently, there is no effective therapeutic available for JE; therefore, finding the effective antiviral agent against JEV replication is crucial. In the...

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Autores principales: Johari, Jefree, Kianmehr, Aynaz, Mustafa, Mohd Rais, Abubakar, Sazaly, Zandi, Keivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546721/
https://www.ncbi.nlm.nih.gov/pubmed/23222683
http://dx.doi.org/10.3390/ijms131216785
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author Johari, Jefree
Kianmehr, Aynaz
Mustafa, Mohd Rais
Abubakar, Sazaly
Zandi, Keivan
author_facet Johari, Jefree
Kianmehr, Aynaz
Mustafa, Mohd Rais
Abubakar, Sazaly
Zandi, Keivan
author_sort Johari, Jefree
collection PubMed
description Japanese encephalitis (JE), a mosquito-borne viral disease, is endemic to the entire east and southeast Asia, and some other parts of the world. Currently, there is no effective therapeutic available for JE; therefore, finding the effective antiviral agent against JEV replication is crucial. In the present study, the in vitro antiviral activity of baicalein and quercetin, two purportedly antiviral bioflavonoids, was evaluated against Japanese encephalitis virus (JEV) replication in Vero cells. Anti-JEV activities of these compounds were examined on different stages of JEV replication cycle. The effects of the compounds on virus replication were determined by foci forming unit reduction assay (FFURA) and quantitative RT-PCR. Baicalein showed potent antiviral activity with IC(50) = 14.28 μg/mL when it was introduced to the Vero cells after adsorption of JEV. Quercetin exhibited weak anti-JEV effects with IC(50) = 212.1 μg/mL when the JEV infected cells were treated with the compound after virus adsorption. However, baicalein exhibited significant effect against JEV adsorption with IC(50) = 7.27 μg/mL while quercetin did not show any anti-adsorption activity. Baicalein also exhibited direct extracellular virucidal activity on JEV with IC(50) = 3.44 μg/mL. However, results of quantitative RT-PCR experiments confirmed the findings from FFURA. This study demonstrated that baicalein should be considered as an appropriate candidate for further investigations, such as the study of molecular and cellular mechanism(s) of action and in vivo evaluation for the development of an effective antiviral compound against Japanese encephalitis virus.
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spelling pubmed-35467212013-01-23 Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus Johari, Jefree Kianmehr, Aynaz Mustafa, Mohd Rais Abubakar, Sazaly Zandi, Keivan Int J Mol Sci Article Japanese encephalitis (JE), a mosquito-borne viral disease, is endemic to the entire east and southeast Asia, and some other parts of the world. Currently, there is no effective therapeutic available for JE; therefore, finding the effective antiviral agent against JEV replication is crucial. In the present study, the in vitro antiviral activity of baicalein and quercetin, two purportedly antiviral bioflavonoids, was evaluated against Japanese encephalitis virus (JEV) replication in Vero cells. Anti-JEV activities of these compounds were examined on different stages of JEV replication cycle. The effects of the compounds on virus replication were determined by foci forming unit reduction assay (FFURA) and quantitative RT-PCR. Baicalein showed potent antiviral activity with IC(50) = 14.28 μg/mL when it was introduced to the Vero cells after adsorption of JEV. Quercetin exhibited weak anti-JEV effects with IC(50) = 212.1 μg/mL when the JEV infected cells were treated with the compound after virus adsorption. However, baicalein exhibited significant effect against JEV adsorption with IC(50) = 7.27 μg/mL while quercetin did not show any anti-adsorption activity. Baicalein also exhibited direct extracellular virucidal activity on JEV with IC(50) = 3.44 μg/mL. However, results of quantitative RT-PCR experiments confirmed the findings from FFURA. This study demonstrated that baicalein should be considered as an appropriate candidate for further investigations, such as the study of molecular and cellular mechanism(s) of action and in vivo evaluation for the development of an effective antiviral compound against Japanese encephalitis virus. Molecular Diversity Preservation International (MDPI) 2012-12-07 /pmc/articles/PMC3546721/ /pubmed/23222683 http://dx.doi.org/10.3390/ijms131216785 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Johari, Jefree
Kianmehr, Aynaz
Mustafa, Mohd Rais
Abubakar, Sazaly
Zandi, Keivan
Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title_full Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title_fullStr Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title_full_unstemmed Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title_short Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus
title_sort antiviral activity of baicalein and quercetin against the japanese encephalitis virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546721/
https://www.ncbi.nlm.nih.gov/pubmed/23222683
http://dx.doi.org/10.3390/ijms131216785
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