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Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus

BACKGROUND: In this study, we developed a SYBR Green-based real-time PCR assay for the detection of sheep pox virus using a plasmid construct carrying one of the highly conserved genes encoding the virion envelope protein (P32) as a template. RESULTS: The method was demonstrated to be highly sensiti...

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Detalles Bibliográficos
Autores principales:	Tian, Hong, Wu, Jingyan, Chen, Yan, Zhang, Keshan, Shang, Youjun, Liu, Xiangtao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546957/ https://www.ncbi.nlm.nih.gov/pubmed/23186407 http://dx.doi.org/10.1186/1743-422X-9-291

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