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Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands
BACKGROUND: Genovar distributions of Chlamydia trachomatis based on ompA typing differ between men who have sex with men (MSM) and heterosexuals. We investigated clonal relationships using a high resolution typing method to characterize C. trachomatis types in these two risk groups. METHODS: C. trac...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547048/ https://www.ncbi.nlm.nih.gov/pubmed/23342025 http://dx.doi.org/10.1371/journal.pone.0053869 |
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author | Bom, Reinier J. M. van der Helm, Jannie J. Schim van der Loeff, Maarten F. van Rooijen, Martijn S. Heijman, Titia Matser, Amy de Vries, Henry J. C. Bruisten, Sylvia M. |
author_facet | Bom, Reinier J. M. van der Helm, Jannie J. Schim van der Loeff, Maarten F. van Rooijen, Martijn S. Heijman, Titia Matser, Amy de Vries, Henry J. C. Bruisten, Sylvia M. |
author_sort | Bom, Reinier J. M. |
collection | PubMed |
description | BACKGROUND: Genovar distributions of Chlamydia trachomatis based on ompA typing differ between men who have sex with men (MSM) and heterosexuals. We investigated clonal relationships using a high resolution typing method to characterize C. trachomatis types in these two risk groups. METHODS: C. trachomatis positive samples were collected at the STI outpatient clinic in Amsterdam between 2008 and 2010 and genotyped by multilocus sequence typing. Clusters were assigned using minimum spanning trees and these were combined with epidemiological data of the hosts. RESULTS: We typed 526 C. trachomatis positive samples: 270 from MSM and 256 from heterosexuals. Eight clusters, containing 10–128 samples were identified of which 4 consisted of samples from MSM (90%–100%), with genovars D, G, J, and L2b. The other 4 clusters consisted mainly of samples from heterosexuals (87%–100%) with genovars D, E, F, I, and J. Genetic diversity was much lower in the MSM clusters than in heterosexual clusters. Significant differences in number of sexual partners and HIV-serostatus were observed for MSM–associated clusters. CONCLUSIONS: C. trachomatis transmission patterns among MSM and heterosexuals were largely distinct. We hypothesize that these differences are due to sexual host behavior, but bacterial factors may play a role as well. |
format | Online Article Text |
id | pubmed-3547048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35470482013-01-22 Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands Bom, Reinier J. M. van der Helm, Jannie J. Schim van der Loeff, Maarten F. van Rooijen, Martijn S. Heijman, Titia Matser, Amy de Vries, Henry J. C. Bruisten, Sylvia M. PLoS One Research Article BACKGROUND: Genovar distributions of Chlamydia trachomatis based on ompA typing differ between men who have sex with men (MSM) and heterosexuals. We investigated clonal relationships using a high resolution typing method to characterize C. trachomatis types in these two risk groups. METHODS: C. trachomatis positive samples were collected at the STI outpatient clinic in Amsterdam between 2008 and 2010 and genotyped by multilocus sequence typing. Clusters were assigned using minimum spanning trees and these were combined with epidemiological data of the hosts. RESULTS: We typed 526 C. trachomatis positive samples: 270 from MSM and 256 from heterosexuals. Eight clusters, containing 10–128 samples were identified of which 4 consisted of samples from MSM (90%–100%), with genovars D, G, J, and L2b. The other 4 clusters consisted mainly of samples from heterosexuals (87%–100%) with genovars D, E, F, I, and J. Genetic diversity was much lower in the MSM clusters than in heterosexual clusters. Significant differences in number of sexual partners and HIV-serostatus were observed for MSM–associated clusters. CONCLUSIONS: C. trachomatis transmission patterns among MSM and heterosexuals were largely distinct. We hypothesize that these differences are due to sexual host behavior, but bacterial factors may play a role as well. Public Library of Science 2013-01-16 /pmc/articles/PMC3547048/ /pubmed/23342025 http://dx.doi.org/10.1371/journal.pone.0053869 Text en © 2013 Bom et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bom, Reinier J. M. van der Helm, Jannie J. Schim van der Loeff, Maarten F. van Rooijen, Martijn S. Heijman, Titia Matser, Amy de Vries, Henry J. C. Bruisten, Sylvia M. Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title | Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title_full | Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title_fullStr | Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title_full_unstemmed | Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title_short | Distinct Transmission Networks of Chlamydia trachomatis in Men Who Have Sex with Men and Heterosexual Adults in Amsterdam, The Netherlands |
title_sort | distinct transmission networks of chlamydia trachomatis in men who have sex with men and heterosexual adults in amsterdam, the netherlands |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547048/ https://www.ncbi.nlm.nih.gov/pubmed/23342025 http://dx.doi.org/10.1371/journal.pone.0053869 |
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