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Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis

BACKGROUND: Fibrocytes are circulating precursors for fibroblasts. Blood fibrocytes are increased in patients with idiopathic pulmonary fibrosis (IPF). The aim of this study was to determine whether alveolar fibrocytes are detected in broncho-alveolar lavage (BAL), to identify their prognostic value...

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Autores principales: Borie, Raphael, Quesnel, Christophe, Phin, Sophie, Debray, Marie-Pierre, Marchal-Somme, Joelle, Tiev, Kiet, Bonay, Marcel, Fabre, Aurélie, Soler, Paul, Dehoux, Monique, Crestani, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547062/
https://www.ncbi.nlm.nih.gov/pubmed/23341987
http://dx.doi.org/10.1371/journal.pone.0053736
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author Borie, Raphael
Quesnel, Christophe
Phin, Sophie
Debray, Marie-Pierre
Marchal-Somme, Joelle
Tiev, Kiet
Bonay, Marcel
Fabre, Aurélie
Soler, Paul
Dehoux, Monique
Crestani, Bruno
author_facet Borie, Raphael
Quesnel, Christophe
Phin, Sophie
Debray, Marie-Pierre
Marchal-Somme, Joelle
Tiev, Kiet
Bonay, Marcel
Fabre, Aurélie
Soler, Paul
Dehoux, Monique
Crestani, Bruno
author_sort Borie, Raphael
collection PubMed
description BACKGROUND: Fibrocytes are circulating precursors for fibroblasts. Blood fibrocytes are increased in patients with idiopathic pulmonary fibrosis (IPF). The aim of this study was to determine whether alveolar fibrocytes are detected in broncho-alveolar lavage (BAL), to identify their prognostic value, and their potential association with culture of fibroblasts from BAL. METHODS: We quantified fibrocytes in BAL from 26 patients with IPF, 9 patients with Systemic Sclerosis(SSc)-interstitial lung disease (ILD), and 11 controls. BAL cells were cultured to isolate alveolar fibroblasts. RESULTS: Fibrocytes were detected in BAL in 14/26 IPF (54%) and 5/9 SSc patients (55%), and never in controls. Fibrocytes were in median 2.5% [0.4–19.7] and 3.0% [2.7–3.7] of BAL cells in IPF and SSc-ILD patients respectively. In IPF patients, the number of alveolar fibrocytes was correlated with the number of alveolar macrophages and was associated with a less severe disease but not with a better outcome. Fibroblasts were cultured from BAL in 12/26 IPF (46%), 5/9 SSc-ILD (65%) and never in controls. The detection of BAL fibrocytes did not predict a positive culture of fibroblasts. CONCLUSION: Fibrocytes were detected in BAL fluid in about half of the patients with IPF and SSc-ILD. Their number was associated with less severe disease in IPF patients and did not associate with the capacity to grow fibroblasts from BAL fluid.
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spelling pubmed-35470622013-01-22 Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis Borie, Raphael Quesnel, Christophe Phin, Sophie Debray, Marie-Pierre Marchal-Somme, Joelle Tiev, Kiet Bonay, Marcel Fabre, Aurélie Soler, Paul Dehoux, Monique Crestani, Bruno PLoS One Research Article BACKGROUND: Fibrocytes are circulating precursors for fibroblasts. Blood fibrocytes are increased in patients with idiopathic pulmonary fibrosis (IPF). The aim of this study was to determine whether alveolar fibrocytes are detected in broncho-alveolar lavage (BAL), to identify their prognostic value, and their potential association with culture of fibroblasts from BAL. METHODS: We quantified fibrocytes in BAL from 26 patients with IPF, 9 patients with Systemic Sclerosis(SSc)-interstitial lung disease (ILD), and 11 controls. BAL cells were cultured to isolate alveolar fibroblasts. RESULTS: Fibrocytes were detected in BAL in 14/26 IPF (54%) and 5/9 SSc patients (55%), and never in controls. Fibrocytes were in median 2.5% [0.4–19.7] and 3.0% [2.7–3.7] of BAL cells in IPF and SSc-ILD patients respectively. In IPF patients, the number of alveolar fibrocytes was correlated with the number of alveolar macrophages and was associated with a less severe disease but not with a better outcome. Fibroblasts were cultured from BAL in 12/26 IPF (46%), 5/9 SSc-ILD (65%) and never in controls. The detection of BAL fibrocytes did not predict a positive culture of fibroblasts. CONCLUSION: Fibrocytes were detected in BAL fluid in about half of the patients with IPF and SSc-ILD. Their number was associated with less severe disease in IPF patients and did not associate with the capacity to grow fibroblasts from BAL fluid. Public Library of Science 2013-01-16 /pmc/articles/PMC3547062/ /pubmed/23341987 http://dx.doi.org/10.1371/journal.pone.0053736 Text en © 2013 Borie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Borie, Raphael
Quesnel, Christophe
Phin, Sophie
Debray, Marie-Pierre
Marchal-Somme, Joelle
Tiev, Kiet
Bonay, Marcel
Fabre, Aurélie
Soler, Paul
Dehoux, Monique
Crestani, Bruno
Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title_full Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title_fullStr Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title_full_unstemmed Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title_short Detection of Alveolar Fibrocytes in Idiopathic Pulmonary Fibrosis and Systemic Sclerosis
title_sort detection of alveolar fibrocytes in idiopathic pulmonary fibrosis and systemic sclerosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547062/
https://www.ncbi.nlm.nih.gov/pubmed/23341987
http://dx.doi.org/10.1371/journal.pone.0053736
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