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Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis

PURPOSE: The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on caveolin 1 in rat urinary bladder and to determine the role of these molecules in the bladder dysfunction that occurs in inflammatory change in rat urinary bladder. METHODS:...

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Autores principales: Kim, Sun-Ouck, Song, Seung Hee, Lee, Seung-Chul, Cho, Kyung A, Yu, Ho Song, Hwang, In Sang, Hwang, Eu Chang, Kwon, Dongdeuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Continence Society 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547177/
https://www.ncbi.nlm.nih.gov/pubmed/23346482
http://dx.doi.org/10.5213/inj.2012.16.4.169
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author Kim, Sun-Ouck
Song, Seung Hee
Lee, Seung-Chul
Cho, Kyung A
Yu, Ho Song
Hwang, In Sang
Hwang, Eu Chang
Kwon, Dongdeuk
author_facet Kim, Sun-Ouck
Song, Seung Hee
Lee, Seung-Chul
Cho, Kyung A
Yu, Ho Song
Hwang, In Sang
Hwang, Eu Chang
Kwon, Dongdeuk
author_sort Kim, Sun-Ouck
collection PubMed
description PURPOSE: The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on caveolin 1 in rat urinary bladder and to determine the role of these molecules in the bladder dysfunction that occurs in inflammatory change in rat urinary bladder. METHODS: Female Sprague-Dawley rats were divided into control (n=30) and experimental (n=30) groups. Cystitis in experimental group was induced by intraperitoneal injection of CYP (200 mg/kg). The control group underwent an intraperitoneal saline injection. After 3 days, urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of caveolin 1 were determined by Western blot and immunofluorescent study in rat urinary bladder. RESULTS: In cystometrograms, the contraction interval (minute) was significantly increased in the CYP-induced cystitis rats (15.8±1.5) than in the control group (6.3±0.5) (P<0.05). Conversely, the average contraction pressure (mmHg) was significantly higher in the CYP-induced cystitis rats (15.6±1.7) than in the control group (11.3±0.5) (P<0.05). Caveolin 1 was expressed in the capillaries, arteriols and venules. The protein expression of caveolin 1 was significantly decreased in the CYP-induced cystitis rats (P<0.05). CONCLUSIONS: Inflammatory change of urinary bladder maybe causes a significant change in the expression of caveolin 1. These findings suggest that caveolin 1 might have a functional role in the bladder dysfunction related with cystitis in rat urinary bladder.
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spelling pubmed-35471772013-01-23 Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis Kim, Sun-Ouck Song, Seung Hee Lee, Seung-Chul Cho, Kyung A Yu, Ho Song Hwang, In Sang Hwang, Eu Chang Kwon, Dongdeuk Int Neurourol J Original Article PURPOSE: The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on caveolin 1 in rat urinary bladder and to determine the role of these molecules in the bladder dysfunction that occurs in inflammatory change in rat urinary bladder. METHODS: Female Sprague-Dawley rats were divided into control (n=30) and experimental (n=30) groups. Cystitis in experimental group was induced by intraperitoneal injection of CYP (200 mg/kg). The control group underwent an intraperitoneal saline injection. After 3 days, urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of caveolin 1 were determined by Western blot and immunofluorescent study in rat urinary bladder. RESULTS: In cystometrograms, the contraction interval (minute) was significantly increased in the CYP-induced cystitis rats (15.8±1.5) than in the control group (6.3±0.5) (P<0.05). Conversely, the average contraction pressure (mmHg) was significantly higher in the CYP-induced cystitis rats (15.6±1.7) than in the control group (11.3±0.5) (P<0.05). Caveolin 1 was expressed in the capillaries, arteriols and venules. The protein expression of caveolin 1 was significantly decreased in the CYP-induced cystitis rats (P<0.05). CONCLUSIONS: Inflammatory change of urinary bladder maybe causes a significant change in the expression of caveolin 1. These findings suggest that caveolin 1 might have a functional role in the bladder dysfunction related with cystitis in rat urinary bladder. Korean Continence Society 2012-12 2012-12-31 /pmc/articles/PMC3547177/ /pubmed/23346482 http://dx.doi.org/10.5213/inj.2012.16.4.169 Text en Copyright © 2012 Korean Continence Society http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Sun-Ouck
Song, Seung Hee
Lee, Seung-Chul
Cho, Kyung A
Yu, Ho Song
Hwang, In Sang
Hwang, Eu Chang
Kwon, Dongdeuk
Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title_full Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title_fullStr Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title_full_unstemmed Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title_short Expression of Caveolin-1 in Rat Urinary Bladder with Cyclophosphamide-Induced Cystitis
title_sort expression of caveolin-1 in rat urinary bladder with cyclophosphamide-induced cystitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547177/
https://www.ncbi.nlm.nih.gov/pubmed/23346482
http://dx.doi.org/10.5213/inj.2012.16.4.169
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