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Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology

We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen...

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Detalles Bibliográficos
Autores principales: Kondo, Kazunari, Uenoyama, Asami, Kitagawa, Ryo, Tsunoda, Hajime, Kusumoto-Matsuo, Rika, Mori, Seiichiro, Ishii, Yoshiyuki, Takeuchi, Takamasa, Kanda, Tadahito, Kukimoto, Iwao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547357/
https://www.ncbi.nlm.nih.gov/pubmed/23341864
http://dx.doi.org/10.2174/1874357901206010277
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author Kondo, Kazunari
Uenoyama, Asami
Kitagawa, Ryo
Tsunoda, Hajime
Kusumoto-Matsuo, Rika
Mori, Seiichiro
Ishii, Yoshiyuki
Takeuchi, Takamasa
Kanda, Tadahito
Kukimoto, Iwao
author_facet Kondo, Kazunari
Uenoyama, Asami
Kitagawa, Ryo
Tsunoda, Hajime
Kusumoto-Matsuo, Rika
Mori, Seiichiro
Ishii, Yoshiyuki
Takeuchi, Takamasa
Kanda, Tadahito
Kukimoto, Iwao
author_sort Kondo, Kazunari
collection PubMed
description We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries.
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spelling pubmed-35473572013-01-22 Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology Kondo, Kazunari Uenoyama, Asami Kitagawa, Ryo Tsunoda, Hajime Kusumoto-Matsuo, Rika Mori, Seiichiro Ishii, Yoshiyuki Takeuchi, Takamasa Kanda, Tadahito Kukimoto, Iwao Open Virol J Article We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries. Bentham Open 2012-12-28 /pmc/articles/PMC3547357/ /pubmed/23341864 http://dx.doi.org/10.2174/1874357901206010277 Text en © Kondo et al.; Licensee Bentham Open. http: //creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Kondo, Kazunari
Uenoyama, Asami
Kitagawa, Ryo
Tsunoda, Hajime
Kusumoto-Matsuo, Rika
Mori, Seiichiro
Ishii, Yoshiyuki
Takeuchi, Takamasa
Kanda, Tadahito
Kukimoto, Iwao
Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title_full Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title_fullStr Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title_full_unstemmed Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title_short Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
title_sort genotype distribution of human papillomaviruses in japanese women with abnormal cervical cytology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547357/
https://www.ncbi.nlm.nih.gov/pubmed/23341864
http://dx.doi.org/10.2174/1874357901206010277
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