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Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites

BACKGROUND: Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s...

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Autores principales: Ali, Zakia M I, Bakli, Mahfoud, Fontaine, Albin, Bakkali, Nawal, Hai, Vinh Vu, Audebert, Stephane, Boublik, Yvan, Pagès, Frederic, Remoué, Franck, Rogier, Christophe, Fraisier, Christophe, Almeras, Lionel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547717/
https://www.ncbi.nlm.nih.gov/pubmed/23276246
http://dx.doi.org/10.1186/1475-2875-11-439
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author Ali, Zakia M I
Bakli, Mahfoud
Fontaine, Albin
Bakkali, Nawal
Hai, Vinh Vu
Audebert, Stephane
Boublik, Yvan
Pagès, Frederic
Remoué, Franck
Rogier, Christophe
Fraisier, Christophe
Almeras, Lionel
author_facet Ali, Zakia M I
Bakli, Mahfoud
Fontaine, Albin
Bakkali, Nawal
Hai, Vinh Vu
Audebert, Stephane
Boublik, Yvan
Pagès, Frederic
Remoué, Franck
Rogier, Christophe
Fraisier, Christophe
Almeras, Lionel
author_sort Ali, Zakia M I
collection PubMed
description BACKGROUND: Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An. gambiae and/or An. funestus bites. METHODS: For this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45). RESULTS: The IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An. gambiae, were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein. CONCLUSION: This study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed.
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spelling pubmed-35477172013-01-23 Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites Ali, Zakia M I Bakli, Mahfoud Fontaine, Albin Bakkali, Nawal Hai, Vinh Vu Audebert, Stephane Boublik, Yvan Pagès, Frederic Remoué, Franck Rogier, Christophe Fraisier, Christophe Almeras, Lionel Malar J Research BACKGROUND: Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An. gambiae and/or An. funestus bites. METHODS: For this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45). RESULTS: The IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An. gambiae, were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein. CONCLUSION: This study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed. BioMed Central 2012-12-31 /pmc/articles/PMC3547717/ /pubmed/23276246 http://dx.doi.org/10.1186/1475-2875-11-439 Text en Copyright ©2012 Ali et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ali, Zakia M I
Bakli, Mahfoud
Fontaine, Albin
Bakkali, Nawal
Hai, Vinh Vu
Audebert, Stephane
Boublik, Yvan
Pagès, Frederic
Remoué, Franck
Rogier, Christophe
Fraisier, Christophe
Almeras, Lionel
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title_full Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title_fullStr Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title_full_unstemmed Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title_short Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
title_sort assessment of anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547717/
https://www.ncbi.nlm.nih.gov/pubmed/23276246
http://dx.doi.org/10.1186/1475-2875-11-439
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