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An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation

BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural ste...

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Autores principales: Liu, Changmei, Teng, Zhao-Qian, McQuate, Andrea L., Jobe, Emily M., Christ, Christa C., von Hoyningen-Huene, Sergei J., Reyes, Marie D., Polich, Eric D., Xing, Yina, Li, Yue, Guo, Weixiang, Zhao, Xinyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547917/
https://www.ncbi.nlm.nih.gov/pubmed/23349673
http://dx.doi.org/10.1371/journal.pone.0051436
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author Liu, Changmei
Teng, Zhao-Qian
McQuate, Andrea L.
Jobe, Emily M.
Christ, Christa C.
von Hoyningen-Huene, Sergei J.
Reyes, Marie D.
Polich, Eric D.
Xing, Yina
Li, Yue
Guo, Weixiang
Zhao, Xinyu
author_facet Liu, Changmei
Teng, Zhao-Qian
McQuate, Andrea L.
Jobe, Emily M.
Christ, Christa C.
von Hoyningen-Huene, Sergei J.
Reyes, Marie D.
Polich, Eric D.
Xing, Yina
Li, Yue
Guo, Weixiang
Zhao, Xinyu
author_sort Liu, Changmei
collection PubMed
description BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural stem/progenitor cells (aNSCs). We recently demonstrated that MBD1 deficiency in aNSCs leads to altered expression of several noncoding microRNAs (miRNAs). METHODOLOGY/PRINCIPAL FINDINGS: Here we show that one of these miRNAs, miR-195, and MBD1 form a negative feedback loop. While MBD1 directly represses the expression of miR-195 in aNSCs, high levels of miR-195 in turn repress the expression of MBD1. Both gain-of-function and loss-of-function investigations show that alterations of the MBD1–miR-195 feedback loop tip the balance between aNSC proliferation and differentiation. CONCLUSIONS/SIGNIFICANCE: Therefore the regulatory loop formed by MBD1 and miR-195 is an important component of the epigenetic network that controls aNSC fate.
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spelling pubmed-35479172013-01-24 An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation Liu, Changmei Teng, Zhao-Qian McQuate, Andrea L. Jobe, Emily M. Christ, Christa C. von Hoyningen-Huene, Sergei J. Reyes, Marie D. Polich, Eric D. Xing, Yina Li, Yue Guo, Weixiang Zhao, Xinyu PLoS One Research Article BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural stem/progenitor cells (aNSCs). We recently demonstrated that MBD1 deficiency in aNSCs leads to altered expression of several noncoding microRNAs (miRNAs). METHODOLOGY/PRINCIPAL FINDINGS: Here we show that one of these miRNAs, miR-195, and MBD1 form a negative feedback loop. While MBD1 directly represses the expression of miR-195 in aNSCs, high levels of miR-195 in turn repress the expression of MBD1. Both gain-of-function and loss-of-function investigations show that alterations of the MBD1–miR-195 feedback loop tip the balance between aNSC proliferation and differentiation. CONCLUSIONS/SIGNIFICANCE: Therefore the regulatory loop formed by MBD1 and miR-195 is an important component of the epigenetic network that controls aNSC fate. Public Library of Science 2013-01-17 /pmc/articles/PMC3547917/ /pubmed/23349673 http://dx.doi.org/10.1371/journal.pone.0051436 Text en © 2013 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Changmei
Teng, Zhao-Qian
McQuate, Andrea L.
Jobe, Emily M.
Christ, Christa C.
von Hoyningen-Huene, Sergei J.
Reyes, Marie D.
Polich, Eric D.
Xing, Yina
Li, Yue
Guo, Weixiang
Zhao, Xinyu
An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title_full An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title_fullStr An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title_full_unstemmed An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title_short An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
title_sort epigenetic feedback regulatory loop involving microrna-195 and mbd1 governs neural stem cell differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547917/
https://www.ncbi.nlm.nih.gov/pubmed/23349673
http://dx.doi.org/10.1371/journal.pone.0051436
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