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An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation
BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural ste...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547917/ https://www.ncbi.nlm.nih.gov/pubmed/23349673 http://dx.doi.org/10.1371/journal.pone.0051436 |
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author | Liu, Changmei Teng, Zhao-Qian McQuate, Andrea L. Jobe, Emily M. Christ, Christa C. von Hoyningen-Huene, Sergei J. Reyes, Marie D. Polich, Eric D. Xing, Yina Li, Yue Guo, Weixiang Zhao, Xinyu |
author_facet | Liu, Changmei Teng, Zhao-Qian McQuate, Andrea L. Jobe, Emily M. Christ, Christa C. von Hoyningen-Huene, Sergei J. Reyes, Marie D. Polich, Eric D. Xing, Yina Li, Yue Guo, Weixiang Zhao, Xinyu |
author_sort | Liu, Changmei |
collection | PubMed |
description | BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural stem/progenitor cells (aNSCs). We recently demonstrated that MBD1 deficiency in aNSCs leads to altered expression of several noncoding microRNAs (miRNAs). METHODOLOGY/PRINCIPAL FINDINGS: Here we show that one of these miRNAs, miR-195, and MBD1 form a negative feedback loop. While MBD1 directly represses the expression of miR-195 in aNSCs, high levels of miR-195 in turn repress the expression of MBD1. Both gain-of-function and loss-of-function investigations show that alterations of the MBD1–miR-195 feedback loop tip the balance between aNSC proliferation and differentiation. CONCLUSIONS/SIGNIFICANCE: Therefore the regulatory loop formed by MBD1 and miR-195 is an important component of the epigenetic network that controls aNSC fate. |
format | Online Article Text |
id | pubmed-3547917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35479172013-01-24 An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation Liu, Changmei Teng, Zhao-Qian McQuate, Andrea L. Jobe, Emily M. Christ, Christa C. von Hoyningen-Huene, Sergei J. Reyes, Marie D. Polich, Eric D. Xing, Yina Li, Yue Guo, Weixiang Zhao, Xinyu PLoS One Research Article BACKGROUND: Epigenetic mechanisms, including DNA methylation, histone modification, and microRNAs, play pivotal roles in stem cell biology. Methyl-CpG binding protein 1 (MBD1), an important epigenetic regulator of adult neurogenesis, controls the proliferation and differentiation of adult neural stem/progenitor cells (aNSCs). We recently demonstrated that MBD1 deficiency in aNSCs leads to altered expression of several noncoding microRNAs (miRNAs). METHODOLOGY/PRINCIPAL FINDINGS: Here we show that one of these miRNAs, miR-195, and MBD1 form a negative feedback loop. While MBD1 directly represses the expression of miR-195 in aNSCs, high levels of miR-195 in turn repress the expression of MBD1. Both gain-of-function and loss-of-function investigations show that alterations of the MBD1–miR-195 feedback loop tip the balance between aNSC proliferation and differentiation. CONCLUSIONS/SIGNIFICANCE: Therefore the regulatory loop formed by MBD1 and miR-195 is an important component of the epigenetic network that controls aNSC fate. Public Library of Science 2013-01-17 /pmc/articles/PMC3547917/ /pubmed/23349673 http://dx.doi.org/10.1371/journal.pone.0051436 Text en © 2013 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Changmei Teng, Zhao-Qian McQuate, Andrea L. Jobe, Emily M. Christ, Christa C. von Hoyningen-Huene, Sergei J. Reyes, Marie D. Polich, Eric D. Xing, Yina Li, Yue Guo, Weixiang Zhao, Xinyu An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title | An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title_full | An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title_fullStr | An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title_full_unstemmed | An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title_short | An Epigenetic Feedback Regulatory Loop Involving MicroRNA-195 and MBD1 Governs Neural Stem Cell Differentiation |
title_sort | epigenetic feedback regulatory loop involving microrna-195 and mbd1 governs neural stem cell differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3547917/ https://www.ncbi.nlm.nih.gov/pubmed/23349673 http://dx.doi.org/10.1371/journal.pone.0051436 |
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