A note on the organization and expression of β-defensin genes in Polish goats

Nucleotide (cds) and amino acids sequences of the caprine β2-defensin genes were in silico compared to search for the sequence variation and for the LAP gene sequences in the goat genome and for the presence of LAP gene transcripts in goat tissues. The comparison of the exon sequences revealed that...

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Autores principales: Bagnicka, E., Prusak, B., Kościuczuk, E., Jarczak, J., Kaba, J., Strzałkowska, N., Jóźwik, A., Czopowicz, M., Krzyżewski, J., Zwierzchowski, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Animal Genetics • Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548089/
https://www.ncbi.nlm.nih.gov/pubmed/23179605
http://dx.doi.org/10.1007/s13353-012-0124-y
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author Bagnicka, E.
Prusak, B.
Kościuczuk, E.
Jarczak, J.
Kaba, J.
Strzałkowska, N.
Jóźwik, A.
Czopowicz, M.
Krzyżewski, J.
Zwierzchowski, L.
author_facet Bagnicka, E.
Prusak, B.
Kościuczuk, E.
Jarczak, J.
Kaba, J.
Strzałkowska, N.
Jóźwik, A.
Czopowicz, M.
Krzyżewski, J.
Zwierzchowski, L.
author_sort Bagnicka, E.
collection PubMed
description Nucleotide (cds) and amino acids sequences of the caprine β2-defensin genes were in silico compared to search for the sequence variation and for the LAP gene sequences in the goat genome and for the presence of LAP gene transcripts in goat tissues. The comparison of the exon sequences revealed that the first 64 amino acids are identical in both LAP and β1-defensin. However, the GBD-1 prepropeptide is shorter by 18 amino acids due to the presence of the stop codon UAA at position 209–211 in GBD-1 mRNA. The LAP gene, which was found, so far, only in Indian goat breeds, is absent in the genome of Polish dairy goats. The introns of the caprine β1- and β2-defensin genes were, for the first time, sequenced; their sequences showed 99.6 % identity, differing in six nucleotide positions.
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spelling pubmed-35480892013-01-18 A note on the organization and expression of β-defensin genes in Polish goats Bagnicka, E. Prusak, B. Kościuczuk, E. Jarczak, J. Kaba, J. Strzałkowska, N. Jóźwik, A. Czopowicz, M. Krzyżewski, J. Zwierzchowski, L. J Appl Genet Animal Genetics • Short Communication Nucleotide (cds) and amino acids sequences of the caprine β2-defensin genes were in silico compared to search for the sequence variation and for the LAP gene sequences in the goat genome and for the presence of LAP gene transcripts in goat tissues. The comparison of the exon sequences revealed that the first 64 amino acids are identical in both LAP and β1-defensin. However, the GBD-1 prepropeptide is shorter by 18 amino acids due to the presence of the stop codon UAA at position 209–211 in GBD-1 mRNA. The LAP gene, which was found, so far, only in Indian goat breeds, is absent in the genome of Polish dairy goats. The introns of the caprine β1- and β2-defensin genes were, for the first time, sequenced; their sequences showed 99.6 % identity, differing in six nucleotide positions. Springer-Verlag 2012-11-22 2013 /pmc/articles/PMC3548089/ /pubmed/23179605 http://dx.doi.org/10.1007/s13353-012-0124-y Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Animal Genetics • Short Communication
Bagnicka, E.
Prusak, B.
Kościuczuk, E.
Jarczak, J.
Kaba, J.
Strzałkowska, N.
Jóźwik, A.
Czopowicz, M.
Krzyżewski, J.
Zwierzchowski, L.
A note on the organization and expression of β-defensin genes in Polish goats
title A note on the organization and expression of β-defensin genes in Polish goats
title_full A note on the organization and expression of β-defensin genes in Polish goats
title_fullStr A note on the organization and expression of β-defensin genes in Polish goats
title_full_unstemmed A note on the organization and expression of β-defensin genes in Polish goats
title_short A note on the organization and expression of β-defensin genes in Polish goats
title_sort note on the organization and expression of β-defensin genes in polish goats
topic Animal Genetics • Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548089/
https://www.ncbi.nlm.nih.gov/pubmed/23179605
http://dx.doi.org/10.1007/s13353-012-0124-y
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