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Cell sorting in a Petri dish controlled by computer vision
Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548191/ https://www.ncbi.nlm.nih.gov/pubmed/23336070 http://dx.doi.org/10.1038/srep01088 |
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author | Környei, Z. Beke, S. Mihálffy, T. Jelitai, M. Kovács, K. J. Szabó, Z. Szabó, B. |
author_facet | Környei, Z. Beke, S. Mihálffy, T. Jelitai, M. Kovács, K. J. Szabó, Z. Szabó, B. |
author_sort | Környei, Z. |
collection | PubMed |
description | Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50–70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66±12% and 88±16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95±2% and 62±7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS. |
format | Online Article Text |
id | pubmed-3548191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35481912013-01-18 Cell sorting in a Petri dish controlled by computer vision Környei, Z. Beke, S. Mihálffy, T. Jelitai, M. Kovács, K. J. Szabó, Z. Szabó, B. Sci Rep Article Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50–70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66±12% and 88±16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95±2% and 62±7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS. Nature Publishing Group 2013-01-18 /pmc/articles/PMC3548191/ /pubmed/23336070 http://dx.doi.org/10.1038/srep01088 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Környei, Z. Beke, S. Mihálffy, T. Jelitai, M. Kovács, K. J. Szabó, Z. Szabó, B. Cell sorting in a Petri dish controlled by computer vision |
title | Cell sorting in a Petri dish controlled by computer vision |
title_full | Cell sorting in a Petri dish controlled by computer vision |
title_fullStr | Cell sorting in a Petri dish controlled by computer vision |
title_full_unstemmed | Cell sorting in a Petri dish controlled by computer vision |
title_short | Cell sorting in a Petri dish controlled by computer vision |
title_sort | cell sorting in a petri dish controlled by computer vision |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548191/ https://www.ncbi.nlm.nih.gov/pubmed/23336070 http://dx.doi.org/10.1038/srep01088 |
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