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Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

BACKGROUND: In a high-throughput environment, to PCR amplify and sequence a large set of viral isolates from populations that are potentially heterogeneous and continuously evolving, the use of degenerate PCR primers is an important strategy. Degenerate primers allow for the PCR amplification of a w...

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Detalles Bibliográficos
Autores principales:	Li, Kelvin, Shrivastava, Susmita, Brownley, Anushka, Katzel, Dan, Bera, Jayati, Nguyen, Anh Thu, Thovarai, Vishal, Halpin, Rebecca, Stockwell, Timothy B
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548747/ https://www.ncbi.nlm.nih.gov/pubmed/23131097 http://dx.doi.org/10.1186/1743-422X-9-261

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