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Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours

Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) afte...

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Autores principales: Lang, Ren, Liu, Guihua, Shi, Yingai, Bharadwaj, Shantaram, Leng, Xiaoyan, Zhou, Xiaobo, Liu, Hong, Atala, Anthony, Zhang, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548815/
https://www.ncbi.nlm.nih.gov/pubmed/23349776
http://dx.doi.org/10.1371/journal.pone.0053980
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author Lang, Ren
Liu, Guihua
Shi, Yingai
Bharadwaj, Shantaram
Leng, Xiaoyan
Zhou, Xiaobo
Liu, Hong
Atala, Anthony
Zhang, Yuanyuan
author_facet Lang, Ren
Liu, Guihua
Shi, Yingai
Bharadwaj, Shantaram
Leng, Xiaoyan
Zhou, Xiaobo
Liu, Hong
Atala, Anthony
Zhang, Yuanyuan
author_sort Lang, Ren
collection PubMed
description Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20–54 years old). About 6×10(4) cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a “rice grain” shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis.
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spelling pubmed-35488152013-01-24 Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours Lang, Ren Liu, Guihua Shi, Yingai Bharadwaj, Shantaram Leng, Xiaoyan Zhou, Xiaobo Liu, Hong Atala, Anthony Zhang, Yuanyuan PLoS One Research Article Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20–54 years old). About 6×10(4) cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a “rice grain” shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis. Public Library of Science 2013-01-18 /pmc/articles/PMC3548815/ /pubmed/23349776 http://dx.doi.org/10.1371/journal.pone.0053980 Text en © 2013 Lang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lang, Ren
Liu, Guihua
Shi, Yingai
Bharadwaj, Shantaram
Leng, Xiaoyan
Zhou, Xiaobo
Liu, Hong
Atala, Anthony
Zhang, Yuanyuan
Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title_full Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title_fullStr Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title_full_unstemmed Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title_short Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
title_sort self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548815/
https://www.ncbi.nlm.nih.gov/pubmed/23349776
http://dx.doi.org/10.1371/journal.pone.0053980
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