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Heterologous viral expression systems in fosmid vectors increase the functional analysis potential of metagenomic libraries

The extraordinary potential of metagenomic functional analyses to identify activities of interest present in uncultured microorganisms has been limited by reduced gene expression in surrogate hosts. We have developed vectors and specialized E. coli strains as improved metagenomic DNA heterologous ex...

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Detalles Bibliográficos
Autores principales: Terrón-González, L., Medina, C., Limón-Mortés, M. C., Santero, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551230/
https://www.ncbi.nlm.nih.gov/pubmed/23346364
http://dx.doi.org/10.1038/srep01107