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Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551806/ https://www.ncbi.nlm.nih.gov/pubmed/23332096 http://dx.doi.org/10.1186/1477-7827-11-4 |
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author | Piromlertamorn, Waraporn Saeng-anan, Ubol Vutyavanich, Teraporn |
author_facet | Piromlertamorn, Waraporn Saeng-anan, Ubol Vutyavanich, Teraporn |
author_sort | Piromlertamorn, Waraporn |
collection | PubMed |
description | BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes to ovarian endometriotic fluid. METHODS: Mouse oocytes/cumulus complexes (n = 862) were divided into 4 groups, and were exposed to endometriotic fluid (group 1), pooled sera from subjects without endometrioma (group 2), phosphate-buffered saline (group 3), and fertilization medium (controls). After five minutes, oocytes were washed and inseminated. Embryo development was observed daily. The quality of hatching blastocysts was assessed by counting the number of inner cell mass (ICM) and trophectoderm (TE) cells. RESULTS: The fertilization, cleavage and blastocyst formation rates in the four groups were not statistically different. The proportions of hatching/hatched blastocysts from fertilized oocytes in groups 1 and 2 were significantly lower than those in group 3 and controls (P = 0.015). Hatching blastocysts from all groups showed no significant difference in the number of ICM and TE cells. CONCLUSIONS: Exposure of mouse oocytes/cumulus complexes to endometriotic fluid had subtle detrimental effects on subsequent blastocyst development. However, one should be cautious in projecting the results of this study to contaminated human oocytes in a clinical setting. |
format | Online Article Text |
id | pubmed-3551806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35518062013-01-24 Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development Piromlertamorn, Waraporn Saeng-anan, Ubol Vutyavanich, Teraporn Reprod Biol Endocrinol Research BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes to ovarian endometriotic fluid. METHODS: Mouse oocytes/cumulus complexes (n = 862) were divided into 4 groups, and were exposed to endometriotic fluid (group 1), pooled sera from subjects without endometrioma (group 2), phosphate-buffered saline (group 3), and fertilization medium (controls). After five minutes, oocytes were washed and inseminated. Embryo development was observed daily. The quality of hatching blastocysts was assessed by counting the number of inner cell mass (ICM) and trophectoderm (TE) cells. RESULTS: The fertilization, cleavage and blastocyst formation rates in the four groups were not statistically different. The proportions of hatching/hatched blastocysts from fertilized oocytes in groups 1 and 2 were significantly lower than those in group 3 and controls (P = 0.015). Hatching blastocysts from all groups showed no significant difference in the number of ICM and TE cells. CONCLUSIONS: Exposure of mouse oocytes/cumulus complexes to endometriotic fluid had subtle detrimental effects on subsequent blastocyst development. However, one should be cautious in projecting the results of this study to contaminated human oocytes in a clinical setting. BioMed Central 2013-01-18 /pmc/articles/PMC3551806/ /pubmed/23332096 http://dx.doi.org/10.1186/1477-7827-11-4 Text en Copyright ©2013 Piromlertamorn et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Piromlertamorn, Waraporn Saeng-anan, Ubol Vutyavanich, Teraporn Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title | Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title_full | Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title_fullStr | Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title_full_unstemmed | Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title_short | Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
title_sort | effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551806/ https://www.ncbi.nlm.nih.gov/pubmed/23332096 http://dx.doi.org/10.1186/1477-7827-11-4 |
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