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Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development

BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes t...

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Autores principales: Piromlertamorn, Waraporn, Saeng-anan, Ubol, Vutyavanich, Teraporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551806/
https://www.ncbi.nlm.nih.gov/pubmed/23332096
http://dx.doi.org/10.1186/1477-7827-11-4
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author Piromlertamorn, Waraporn
Saeng-anan, Ubol
Vutyavanich, Teraporn
author_facet Piromlertamorn, Waraporn
Saeng-anan, Ubol
Vutyavanich, Teraporn
author_sort Piromlertamorn, Waraporn
collection PubMed
description BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes to ovarian endometriotic fluid. METHODS: Mouse oocytes/cumulus complexes (n = 862) were divided into 4 groups, and were exposed to endometriotic fluid (group 1), pooled sera from subjects without endometrioma (group 2), phosphate-buffered saline (group 3), and fertilization medium (controls). After five minutes, oocytes were washed and inseminated. Embryo development was observed daily. The quality of hatching blastocysts was assessed by counting the number of inner cell mass (ICM) and trophectoderm (TE) cells. RESULTS: The fertilization, cleavage and blastocyst formation rates in the four groups were not statistically different. The proportions of hatching/hatched blastocysts from fertilized oocytes in groups 1 and 2 were significantly lower than those in group 3 and controls (P = 0.015). Hatching blastocysts from all groups showed no significant difference in the number of ICM and TE cells. CONCLUSIONS: Exposure of mouse oocytes/cumulus complexes to endometriotic fluid had subtle detrimental effects on subsequent blastocyst development. However, one should be cautious in projecting the results of this study to contaminated human oocytes in a clinical setting.
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spelling pubmed-35518062013-01-24 Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development Piromlertamorn, Waraporn Saeng-anan, Ubol Vutyavanich, Teraporn Reprod Biol Endocrinol Research BACKGROUND: Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes to ovarian endometriotic fluid. METHODS: Mouse oocytes/cumulus complexes (n = 862) were divided into 4 groups, and were exposed to endometriotic fluid (group 1), pooled sera from subjects without endometrioma (group 2), phosphate-buffered saline (group 3), and fertilization medium (controls). After five minutes, oocytes were washed and inseminated. Embryo development was observed daily. The quality of hatching blastocysts was assessed by counting the number of inner cell mass (ICM) and trophectoderm (TE) cells. RESULTS: The fertilization, cleavage and blastocyst formation rates in the four groups were not statistically different. The proportions of hatching/hatched blastocysts from fertilized oocytes in groups 1 and 2 were significantly lower than those in group 3 and controls (P = 0.015). Hatching blastocysts from all groups showed no significant difference in the number of ICM and TE cells. CONCLUSIONS: Exposure of mouse oocytes/cumulus complexes to endometriotic fluid had subtle detrimental effects on subsequent blastocyst development. However, one should be cautious in projecting the results of this study to contaminated human oocytes in a clinical setting. BioMed Central 2013-01-18 /pmc/articles/PMC3551806/ /pubmed/23332096 http://dx.doi.org/10.1186/1477-7827-11-4 Text en Copyright ©2013 Piromlertamorn et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Piromlertamorn, Waraporn
Saeng-anan, Ubol
Vutyavanich, Teraporn
Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title_full Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title_fullStr Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title_full_unstemmed Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title_short Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
title_sort effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551806/
https://www.ncbi.nlm.nih.gov/pubmed/23332096
http://dx.doi.org/10.1186/1477-7827-11-4
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