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SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition

PURPOSES: The objective of this study was to investigate the role of stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, on bone healing and whether SDF-1 contributes to accelerating bone repair in traumatic brain injury (TBI)/fracture model. MATERIALS AND METHODS: Real-time polymerase ch...

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Autores principales: Liu, Xiaoqi, Zhou, Changlong, Li, Yanjing, Ji, Ye, Xu, Gongping, Wang, Xintao, Yan, Jinglong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551938/
https://www.ncbi.nlm.nih.gov/pubmed/23349789
http://dx.doi.org/10.1371/journal.pone.0054077
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author Liu, Xiaoqi
Zhou, Changlong
Li, Yanjing
Ji, Ye
Xu, Gongping
Wang, Xintao
Yan, Jinglong
author_facet Liu, Xiaoqi
Zhou, Changlong
Li, Yanjing
Ji, Ye
Xu, Gongping
Wang, Xintao
Yan, Jinglong
author_sort Liu, Xiaoqi
collection PubMed
description PURPOSES: The objective of this study was to investigate the role of stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, on bone healing and whether SDF-1 contributes to accelerating bone repair in traumatic brain injury (TBI)/fracture model. MATERIALS AND METHODS: Real-time polymerase chain reaction and immunohistochemical analysis were used to detect the expression of SDF-1 during the repair of femoral bone in TBI/fracture model. The TBI/fracture model was treated with anti–SDF-1 neutralizing antibody or AMD3100, an antagonist for CXCR4, and evaluated by histomorphometry. In vitro and in vivo migration assays were used to evaluate the functional effect of SDF-1 on primary mesenchymal stem cells. RESULTS: The expression of SDF1 and CXCR4 messenger RNA was increased during the bone healing in TBI/fracture model but was less increased in fracture only model. High expression of SDF-1 protein was observed in the surrounding tissue of the damaged bone. Treated with anti–SDF-1 antibody or AMD3100 could inhibit new bone formation. SDF-1 increased mesenchymal stem cell chemotaxis in vitro in a dose-dependent manner. The in vivo migration study demonstrated that mesenchymal stem cells recruited by SDF-1 participate in endochondral bone repair. CONCLUSION: The SDF-1/CXCR4 axis plays a crucial role in the accelerating fracture healing under the condition of TBI and contributes to endochondral bone repair.
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spelling pubmed-35519382013-01-24 SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition Liu, Xiaoqi Zhou, Changlong Li, Yanjing Ji, Ye Xu, Gongping Wang, Xintao Yan, Jinglong PLoS One Research Article PURPOSES: The objective of this study was to investigate the role of stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, on bone healing and whether SDF-1 contributes to accelerating bone repair in traumatic brain injury (TBI)/fracture model. MATERIALS AND METHODS: Real-time polymerase chain reaction and immunohistochemical analysis were used to detect the expression of SDF-1 during the repair of femoral bone in TBI/fracture model. The TBI/fracture model was treated with anti–SDF-1 neutralizing antibody or AMD3100, an antagonist for CXCR4, and evaluated by histomorphometry. In vitro and in vivo migration assays were used to evaluate the functional effect of SDF-1 on primary mesenchymal stem cells. RESULTS: The expression of SDF1 and CXCR4 messenger RNA was increased during the bone healing in TBI/fracture model but was less increased in fracture only model. High expression of SDF-1 protein was observed in the surrounding tissue of the damaged bone. Treated with anti–SDF-1 antibody or AMD3100 could inhibit new bone formation. SDF-1 increased mesenchymal stem cell chemotaxis in vitro in a dose-dependent manner. The in vivo migration study demonstrated that mesenchymal stem cells recruited by SDF-1 participate in endochondral bone repair. CONCLUSION: The SDF-1/CXCR4 axis plays a crucial role in the accelerating fracture healing under the condition of TBI and contributes to endochondral bone repair. Public Library of Science 2013-01-22 /pmc/articles/PMC3551938/ /pubmed/23349789 http://dx.doi.org/10.1371/journal.pone.0054077 Text en © 2013 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Xiaoqi
Zhou, Changlong
Li, Yanjing
Ji, Ye
Xu, Gongping
Wang, Xintao
Yan, Jinglong
SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title_full SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title_fullStr SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title_full_unstemmed SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title_short SDF-1 Promotes Endochondral Bone Repair during Fracture Healing at the Traumatic Brain Injury Condition
title_sort sdf-1 promotes endochondral bone repair during fracture healing at the traumatic brain injury condition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3551938/
https://www.ncbi.nlm.nih.gov/pubmed/23349789
http://dx.doi.org/10.1371/journal.pone.0054077
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