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Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma

The incidence of hepatocellular carcinoma (HCC) in the United States is increasing and the increase is projected to continue for several decades. The overall survival of HCC patients is poor and treatments are not effective in part because most of the diagnoses come at a late stage. The development...

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Detalles Bibliográficos
Autores principales: An, Yanming, Bekesova, Slavka, Edwards, Nathan, Goldman, Radoslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552294/
https://www.ncbi.nlm.nih.gov/pubmed/20826913
http://dx.doi.org/10.3233/DMA-2010-0721
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author An, Yanming
Bekesova, Slavka
Edwards, Nathan
Goldman, Radoslav
author_facet An, Yanming
Bekesova, Slavka
Edwards, Nathan
Goldman, Radoslav
author_sort An, Yanming
collection PubMed
description The incidence of hepatocellular carcinoma (HCC) in the United States is increasing and the increase is projected to continue for several decades. The overall survival of HCC patients is poor and treatments are not effective in part because most of the diagnoses come at a late stage. The development of new markers for detection of HCC would significantly improve patient prognosis. This paper describes identification of candidate markers previously reported in our serologic study of an Egyptian population by quantitative comparison of matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectra. To identify these marker candidates, we performed LC-MS/MS sequencing that identified nine native peptides associated with HCC, including two reported previously. Four truncations of N terminus of complement C3f and a fibrinopeptide increased in control sera; two complement C4α peptides, a zyxin peptide, and a coagulation factor XIII peptide increased in cancer patient sera. We have also identified increased biliverdin diglucuronide in the sera of cancer patients. These peptides could potentially serve as markers of HCC following additional validation studies; however, association of similar peptides with other diseases and cancers dictates a very cautious approach.
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spelling pubmed-35522942013-01-23 Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma An, Yanming Bekesova, Slavka Edwards, Nathan Goldman, Radoslav Dis Markers Other The incidence of hepatocellular carcinoma (HCC) in the United States is increasing and the increase is projected to continue for several decades. The overall survival of HCC patients is poor and treatments are not effective in part because most of the diagnoses come at a late stage. The development of new markers for detection of HCC would significantly improve patient prognosis. This paper describes identification of candidate markers previously reported in our serologic study of an Egyptian population by quantitative comparison of matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectra. To identify these marker candidates, we performed LC-MS/MS sequencing that identified nine native peptides associated with HCC, including two reported previously. Four truncations of N terminus of complement C3f and a fibrinopeptide increased in control sera; two complement C4α peptides, a zyxin peptide, and a coagulation factor XIII peptide increased in cancer patient sera. We have also identified increased biliverdin diglucuronide in the sera of cancer patients. These peptides could potentially serve as markers of HCC following additional validation studies; however, association of similar peptides with other diseases and cancers dictates a very cautious approach. IOS Press 2010 2010-09-08 /pmc/articles/PMC3552294/ /pubmed/20826913 http://dx.doi.org/10.3233/DMA-2010-0721 Text en Copyright © 2010 Hindawi Publishing Corporation.
spellingShingle Other
An, Yanming
Bekesova, Slavka
Edwards, Nathan
Goldman, Radoslav
Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title_full Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title_fullStr Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title_full_unstemmed Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title_short Peptides in Low Molecular Weight Fraction of Serum Associated with Hepatocellular Carcinoma
title_sort peptides in low molecular weight fraction of serum associated with hepatocellular carcinoma
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552294/
https://www.ncbi.nlm.nih.gov/pubmed/20826913
http://dx.doi.org/10.3233/DMA-2010-0721
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