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Exercise training prevents skeletal muscle damage in an experimental sepsis model

OBJECTIVE: Oxidative stress plays an important role in skeletal muscle damage in sepsis. Aerobic exercise can decrease oxidative stress and enhance antioxidant defenses. Therefore, it was hypothesized that aerobic exercise training before a sepsis stimulus could attenuate skeletal muscle damage by m...

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Autores principales: Coelho, Carla Werlang, Jannig, Paulo R, de Souza, Arlete B, Fronza, Hercilio, Westphal, Glauco A, Petronilho, Fabricia, Constantino, Larissa, Dal-Pizzol, Felipe, Ferreira, Gabriela K, Streck, Emilio E, Silva, Eliezer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552448/
https://www.ncbi.nlm.nih.gov/pubmed/23420166
http://dx.doi.org/10.6061/clinics/2013(01)OA17
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author Coelho, Carla Werlang
Jannig, Paulo R
de Souza, Arlete B
Fronza, Hercilio
Westphal, Glauco A
Petronilho, Fabricia
Constantino, Larissa
Dal-Pizzol, Felipe
Ferreira, Gabriela K
Streck, Emilio E
Silva, Eliezer
author_facet Coelho, Carla Werlang
Jannig, Paulo R
de Souza, Arlete B
Fronza, Hercilio
Westphal, Glauco A
Petronilho, Fabricia
Constantino, Larissa
Dal-Pizzol, Felipe
Ferreira, Gabriela K
Streck, Emilio E
Silva, Eliezer
author_sort Coelho, Carla Werlang
collection PubMed
description OBJECTIVE: Oxidative stress plays an important role in skeletal muscle damage in sepsis. Aerobic exercise can decrease oxidative stress and enhance antioxidant defenses. Therefore, it was hypothesized that aerobic exercise training before a sepsis stimulus could attenuate skeletal muscle damage by modulating oxidative stress. Thus, the aim of this study was to evaluate the effects of aerobic physical preconditioning on the different mechanisms that are involved in sepsis-induced myopathy. METHODS: Male Wistar rats were randomly assigned to either the untrained or trained group. The exercise training protocol consisted of an eight-week treadmill program. After the training protocol, the animals from both groups were randomly assigned to either a sham group or a cecal ligation and perforation surgery group. Thus, the groups were as follows: sham, cecal ligation and perforation, sham trained, and cecal ligation and perforation trained. Five days after surgery, the animals were euthanized and their soleus and plantaris muscles were harvested. Fiber cross-sectional area, creatine kinase, thiobarbituric acid reactive species, carbonyl, catalase and superoxide dismutase activities were measured. RESULTS: The fiber cross-sectional area was smaller, and the creatine kinase, thiobarbituric acid reactive species and carbonyl levels were higher in both muscles in the cecal ligation and perforation group than in the sham and cecal ligation and perforation trained groups. The muscle superoxide dismutase activity was higher in the cecal ligation and perforation trained group than in the sham and cecal ligation and perforation groups. The muscle catalase activity was lower in the cecal ligation and perforation group than in the sham group. CONCLUSION: In summary, aerobic physical preconditioning prevents atrophy, lipid peroxidation and protein oxidation and improves superoxide dismutase activity in the skeletal muscles of septic rats.
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spelling pubmed-35524482013-01-28 Exercise training prevents skeletal muscle damage in an experimental sepsis model Coelho, Carla Werlang Jannig, Paulo R de Souza, Arlete B Fronza, Hercilio Westphal, Glauco A Petronilho, Fabricia Constantino, Larissa Dal-Pizzol, Felipe Ferreira, Gabriela K Streck, Emilio E Silva, Eliezer Clinics (Sao Paulo) Basic Research OBJECTIVE: Oxidative stress plays an important role in skeletal muscle damage in sepsis. Aerobic exercise can decrease oxidative stress and enhance antioxidant defenses. Therefore, it was hypothesized that aerobic exercise training before a sepsis stimulus could attenuate skeletal muscle damage by modulating oxidative stress. Thus, the aim of this study was to evaluate the effects of aerobic physical preconditioning on the different mechanisms that are involved in sepsis-induced myopathy. METHODS: Male Wistar rats were randomly assigned to either the untrained or trained group. The exercise training protocol consisted of an eight-week treadmill program. After the training protocol, the animals from both groups were randomly assigned to either a sham group or a cecal ligation and perforation surgery group. Thus, the groups were as follows: sham, cecal ligation and perforation, sham trained, and cecal ligation and perforation trained. Five days after surgery, the animals were euthanized and their soleus and plantaris muscles were harvested. Fiber cross-sectional area, creatine kinase, thiobarbituric acid reactive species, carbonyl, catalase and superoxide dismutase activities were measured. RESULTS: The fiber cross-sectional area was smaller, and the creatine kinase, thiobarbituric acid reactive species and carbonyl levels were higher in both muscles in the cecal ligation and perforation group than in the sham and cecal ligation and perforation trained groups. The muscle superoxide dismutase activity was higher in the cecal ligation and perforation trained group than in the sham and cecal ligation and perforation groups. The muscle catalase activity was lower in the cecal ligation and perforation group than in the sham group. CONCLUSION: In summary, aerobic physical preconditioning prevents atrophy, lipid peroxidation and protein oxidation and improves superoxide dismutase activity in the skeletal muscles of septic rats. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013-01 /pmc/articles/PMC3552448/ /pubmed/23420166 http://dx.doi.org/10.6061/clinics/2013(01)OA17 Text en Copyright © 2013 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Coelho, Carla Werlang
Jannig, Paulo R
de Souza, Arlete B
Fronza, Hercilio
Westphal, Glauco A
Petronilho, Fabricia
Constantino, Larissa
Dal-Pizzol, Felipe
Ferreira, Gabriela K
Streck, Emilio E
Silva, Eliezer
Exercise training prevents skeletal muscle damage in an experimental sepsis model
title Exercise training prevents skeletal muscle damage in an experimental sepsis model
title_full Exercise training prevents skeletal muscle damage in an experimental sepsis model
title_fullStr Exercise training prevents skeletal muscle damage in an experimental sepsis model
title_full_unstemmed Exercise training prevents skeletal muscle damage in an experimental sepsis model
title_short Exercise training prevents skeletal muscle damage in an experimental sepsis model
title_sort exercise training prevents skeletal muscle damage in an experimental sepsis model
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552448/
https://www.ncbi.nlm.nih.gov/pubmed/23420166
http://dx.doi.org/10.6061/clinics/2013(01)OA17
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