Cargando…

Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection

BACKGROUND: High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhao, Theurkauf, William E, Weng, Zhiping, Zamore, Phillip D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552703/
https://www.ncbi.nlm.nih.gov/pubmed/23273270
http://dx.doi.org/10.1186/1758-907X-3-9