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Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
BACKGROUND: High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552703/ https://www.ncbi.nlm.nih.gov/pubmed/23273270 http://dx.doi.org/10.1186/1758-907X-3-9 |