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Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts

The increasing availability of human cardiac tissues for study are critically important in increasing our understanding of the impact of gender, age, and other parameters, such as medications and cardiac disease, on arrhythmia susceptibility. In this study, we aimed to compare the mRNA expression of...

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Autores principales: Ambrosi, Christina M., Yamada, Kathryn A., Nerbonne, Jeanne M., Efimov, Igor R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552854/
https://www.ncbi.nlm.nih.gov/pubmed/23355885
http://dx.doi.org/10.1371/journal.pone.0054635
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author Ambrosi, Christina M.
Yamada, Kathryn A.
Nerbonne, Jeanne M.
Efimov, Igor R.
author_facet Ambrosi, Christina M.
Yamada, Kathryn A.
Nerbonne, Jeanne M.
Efimov, Igor R.
author_sort Ambrosi, Christina M.
collection PubMed
description The increasing availability of human cardiac tissues for study are critically important in increasing our understanding of the impact of gender, age, and other parameters, such as medications and cardiac disease, on arrhythmia susceptibility. In this study, we aimed to compare the mRNA expression of 89 ion channel subunits, calcium handling proteins, and transcription factors important in cardiac conduction and arrhythmogenesis in the left atria (LA) and ventricles (LV) of failing and nonfailing human hearts of both genders. Total RNA samples, prepared from failing male (n = 9) and female (n = 7), and from nonfailing male (n = 9) and female (n = 9) hearts, were probed using custom-designed Taqman gene arrays. Analyses were performed to explore the relationships between gender, failure state, and chamber expression. Hierarchical cluster analysis revealed chamber specific expression patterns, but failed to identify disease- or gender-dependent clustering. Gender-specific analysis showed lower expression levels in transcripts encoding for K(v)4.3, KChIP2, K(v)1.5, and K(ir)3.1 in the failing female as compared with the male LA. Analysis of LV transcripts, however, did not reveal significant differences based on gender. Overall, our data highlight the differential expression and transcriptional remodeling of ion channel subunits in the human heart as a function of gender and cardiac disease. Furthermore, the availability of such data sets will allow for the development of disease-, gender-, and, most importantly, patient-specific cardiac models, with the ability to utilize such information as mRNA expression to predict cardiac phenotype.
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spelling pubmed-35528542013-01-25 Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts Ambrosi, Christina M. Yamada, Kathryn A. Nerbonne, Jeanne M. Efimov, Igor R. PLoS One Research Article The increasing availability of human cardiac tissues for study are critically important in increasing our understanding of the impact of gender, age, and other parameters, such as medications and cardiac disease, on arrhythmia susceptibility. In this study, we aimed to compare the mRNA expression of 89 ion channel subunits, calcium handling proteins, and transcription factors important in cardiac conduction and arrhythmogenesis in the left atria (LA) and ventricles (LV) of failing and nonfailing human hearts of both genders. Total RNA samples, prepared from failing male (n = 9) and female (n = 7), and from nonfailing male (n = 9) and female (n = 9) hearts, were probed using custom-designed Taqman gene arrays. Analyses were performed to explore the relationships between gender, failure state, and chamber expression. Hierarchical cluster analysis revealed chamber specific expression patterns, but failed to identify disease- or gender-dependent clustering. Gender-specific analysis showed lower expression levels in transcripts encoding for K(v)4.3, KChIP2, K(v)1.5, and K(ir)3.1 in the failing female as compared with the male LA. Analysis of LV transcripts, however, did not reveal significant differences based on gender. Overall, our data highlight the differential expression and transcriptional remodeling of ion channel subunits in the human heart as a function of gender and cardiac disease. Furthermore, the availability of such data sets will allow for the development of disease-, gender-, and, most importantly, patient-specific cardiac models, with the ability to utilize such information as mRNA expression to predict cardiac phenotype. Public Library of Science 2013-01-23 /pmc/articles/PMC3552854/ /pubmed/23355885 http://dx.doi.org/10.1371/journal.pone.0054635 Text en © 2013 Ambrosi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ambrosi, Christina M.
Yamada, Kathryn A.
Nerbonne, Jeanne M.
Efimov, Igor R.
Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title_full Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title_fullStr Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title_full_unstemmed Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title_short Gender Differences in Electrophysiological Gene Expression in Failing and Non-Failing Human Hearts
title_sort gender differences in electrophysiological gene expression in failing and non-failing human hearts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3552854/
https://www.ncbi.nlm.nih.gov/pubmed/23355885
http://dx.doi.org/10.1371/journal.pone.0054635
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