UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions

Repairing damaged DNA is essential for an organism’s survival. UV damage endonuclease (UVDE) is a DNA-repair enzyme that can recognize and incise different types of damaged DNA. We present the structure of Sulfolobus acidocaldarius UVDE on its own and in a pre-catalytic complex with UV-damaged DNA c...

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Autores principales: Meulenbroek, Elisabeth M., Peron Cane, Caroline, Jala, Isabelle, Iwai, Shigenori, Moolenaar, Geri F., Goosen, Nora, Pannu, Navraj S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553973/
https://www.ncbi.nlm.nih.gov/pubmed/23221644
http://dx.doi.org/10.1093/nar/gks1127
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author Meulenbroek, Elisabeth M.
Peron Cane, Caroline
Jala, Isabelle
Iwai, Shigenori
Moolenaar, Geri F.
Goosen, Nora
Pannu, Navraj S.
author_facet Meulenbroek, Elisabeth M.
Peron Cane, Caroline
Jala, Isabelle
Iwai, Shigenori
Moolenaar, Geri F.
Goosen, Nora
Pannu, Navraj S.
author_sort Meulenbroek, Elisabeth M.
collection PubMed
description Repairing damaged DNA is essential for an organism’s survival. UV damage endonuclease (UVDE) is a DNA-repair enzyme that can recognize and incise different types of damaged DNA. We present the structure of Sulfolobus acidocaldarius UVDE on its own and in a pre-catalytic complex with UV-damaged DNA containing a 6-4 photoproduct showing a novel ‘dual dinucleotide flip’ mechanism for recognition of damaged dipyrimidines: the two purines opposite to the damaged pyrimidine bases are flipped into a dipurine-specific pocket, while the damaged bases are also flipped into another cleft.
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spelling pubmed-35539732013-01-24 UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions Meulenbroek, Elisabeth M. Peron Cane, Caroline Jala, Isabelle Iwai, Shigenori Moolenaar, Geri F. Goosen, Nora Pannu, Navraj S. Nucleic Acids Res Structural Biology Repairing damaged DNA is essential for an organism’s survival. UV damage endonuclease (UVDE) is a DNA-repair enzyme that can recognize and incise different types of damaged DNA. We present the structure of Sulfolobus acidocaldarius UVDE on its own and in a pre-catalytic complex with UV-damaged DNA containing a 6-4 photoproduct showing a novel ‘dual dinucleotide flip’ mechanism for recognition of damaged dipyrimidines: the two purines opposite to the damaged pyrimidine bases are flipped into a dipurine-specific pocket, while the damaged bases are also flipped into another cleft. Oxford University Press 2013-01 2012-12-11 /pmc/articles/PMC3553973/ /pubmed/23221644 http://dx.doi.org/10.1093/nar/gks1127 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Structural Biology
Meulenbroek, Elisabeth M.
Peron Cane, Caroline
Jala, Isabelle
Iwai, Shigenori
Moolenaar, Geri F.
Goosen, Nora
Pannu, Navraj S.
UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title_full UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title_fullStr UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title_full_unstemmed UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title_short UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions
title_sort uv damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different dna lesions
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553973/
https://www.ncbi.nlm.nih.gov/pubmed/23221644
http://dx.doi.org/10.1093/nar/gks1127
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