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Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats

BACKGROUND: Occupational and environmental exposures to lead remain a public health problem as lead alters physiological processes by inducing oxidative stress and mimicking divalent cations. This study was designed to investigate the effects of Vitamin C (VC) and Vitamin E (VE) on the reproductive...

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Autores principales: Ayinde, Oluseyi C, Ogunnowo, Sunday, Ogedegbe, Rita A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554483/
https://www.ncbi.nlm.nih.gov/pubmed/23241495
http://dx.doi.org/10.1186/2050-6511-13-17
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author Ayinde, Oluseyi C
Ogunnowo, Sunday
Ogedegbe, Rita A
author_facet Ayinde, Oluseyi C
Ogunnowo, Sunday
Ogedegbe, Rita A
author_sort Ayinde, Oluseyi C
collection PubMed
description BACKGROUND: Occupational and environmental exposures to lead remain a public health problem as lead alters physiological processes by inducing oxidative stress and mimicking divalent cations. This study was designed to investigate the effects of Vitamin C (VC) and Vitamin E (VE) on the reproductive function of lead exposed male rats. Experimental animals were exposed to oral doses of lead, VC and VE at 60 mg/kg body weight, 40 mg/kg body weight, and 150 mg/kg body weight respectively, while control animals received 0.9% saline solution. Oral administration spanned for six weeks after which changes in testicular redox status, lead deposition, testicular zinc content, serum androgen content, semen quality and testis histology were examined. RESULTS: There were significant (p < 0.05) increases in oxidative stress indices and testicular lead content. A significant (p < 0.05) depletion of zinc in the testis of lead exposed animals was also observed. Fluctuations were observed in androgen levels of lead treated animals with a significant increase (p < 0.05) in Serum follicle stimulating hormone (FSH) and testosterone (TT) content, while there was no significant change in luteinizing hormone (LH) content. Testicular tissue showed an alteration in its normal histology with degeneration of the seminiferous epithelium accompanied by a significant reduction (p < 0.05) in the number of luminal spermatozoa. A downgrade in the semen appearance and semen quality –sperm motility, morphology, and count was also observed after lead exposure. VC and VE treatment showed a significant (p < 0.05) reversal of the physiological alteration induced by lead. CONCLUSIONS: Lead exposure resulted in a decline in the reproductive function of male rats by inducing oxidative stress, inhibiting enzymes and depleting testicular zinc contents. However, results clearly showed that VC and VE attenuated the deleterious impact of lead on the reproductive system.
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spelling pubmed-35544832013-01-29 Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats Ayinde, Oluseyi C Ogunnowo, Sunday Ogedegbe, Rita A BMC Pharmacol Toxicol Research Article BACKGROUND: Occupational and environmental exposures to lead remain a public health problem as lead alters physiological processes by inducing oxidative stress and mimicking divalent cations. This study was designed to investigate the effects of Vitamin C (VC) and Vitamin E (VE) on the reproductive function of lead exposed male rats. Experimental animals were exposed to oral doses of lead, VC and VE at 60 mg/kg body weight, 40 mg/kg body weight, and 150 mg/kg body weight respectively, while control animals received 0.9% saline solution. Oral administration spanned for six weeks after which changes in testicular redox status, lead deposition, testicular zinc content, serum androgen content, semen quality and testis histology were examined. RESULTS: There were significant (p < 0.05) increases in oxidative stress indices and testicular lead content. A significant (p < 0.05) depletion of zinc in the testis of lead exposed animals was also observed. Fluctuations were observed in androgen levels of lead treated animals with a significant increase (p < 0.05) in Serum follicle stimulating hormone (FSH) and testosterone (TT) content, while there was no significant change in luteinizing hormone (LH) content. Testicular tissue showed an alteration in its normal histology with degeneration of the seminiferous epithelium accompanied by a significant reduction (p < 0.05) in the number of luminal spermatozoa. A downgrade in the semen appearance and semen quality –sperm motility, morphology, and count was also observed after lead exposure. VC and VE treatment showed a significant (p < 0.05) reversal of the physiological alteration induced by lead. CONCLUSIONS: Lead exposure resulted in a decline in the reproductive function of male rats by inducing oxidative stress, inhibiting enzymes and depleting testicular zinc contents. However, results clearly showed that VC and VE attenuated the deleterious impact of lead on the reproductive system. BioMed Central 2012-12-14 /pmc/articles/PMC3554483/ /pubmed/23241495 http://dx.doi.org/10.1186/2050-6511-13-17 Text en Copyright ©2012 Ayinde et al. licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ayinde, Oluseyi C
Ogunnowo, Sunday
Ogedegbe, Rita A
Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title_full Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title_fullStr Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title_full_unstemmed Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title_short Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats
title_sort influence of vitamin c and vitamin e on testicular zinc content and testicular toxicity in lead exposed albino rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554483/
https://www.ncbi.nlm.nih.gov/pubmed/23241495
http://dx.doi.org/10.1186/2050-6511-13-17
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