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Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold

BACKGROUND: Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system...

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Autores principales: Klangjorhor, Jeerawan, Nimkingratana, Puwapong, Settakorn, Jongkolnee, Pruksakorn, Dumnoensun, Leerapun, Taninnit, Arpornchayanon, Olarn, Rojanasthien, Sattaya, Kongtawelert, Prachya, Pothacharoen, Peraphan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554561/
https://www.ncbi.nlm.nih.gov/pubmed/23253362
http://dx.doi.org/10.1186/1749-799X-7-40
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author Klangjorhor, Jeerawan
Nimkingratana, Puwapong
Settakorn, Jongkolnee
Pruksakorn, Dumnoensun
Leerapun, Taninnit
Arpornchayanon, Olarn
Rojanasthien, Sattaya
Kongtawelert, Prachya
Pothacharoen, Peraphan
author_facet Klangjorhor, Jeerawan
Nimkingratana, Puwapong
Settakorn, Jongkolnee
Pruksakorn, Dumnoensun
Leerapun, Taninnit
Arpornchayanon, Olarn
Rojanasthien, Sattaya
Kongtawelert, Prachya
Pothacharoen, Peraphan
author_sort Klangjorhor, Jeerawan
collection PubMed
description BACKGROUND: Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done. METHODS: We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR. RESULTS: The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels. CONCLUSIONS: This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture.
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spelling pubmed-35545612013-01-29 Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold Klangjorhor, Jeerawan Nimkingratana, Puwapong Settakorn, Jongkolnee Pruksakorn, Dumnoensun Leerapun, Taninnit Arpornchayanon, Olarn Rojanasthien, Sattaya Kongtawelert, Prachya Pothacharoen, Peraphan J Orthop Surg Res Research Article BACKGROUND: Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done. METHODS: We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR. RESULTS: The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels. CONCLUSIONS: This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture. BioMed Central 2012-12-19 /pmc/articles/PMC3554561/ /pubmed/23253362 http://dx.doi.org/10.1186/1749-799X-7-40 Text en Copyright ©2012 Klangjorhor et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Klangjorhor, Jeerawan
Nimkingratana, Puwapong
Settakorn, Jongkolnee
Pruksakorn, Dumnoensun
Leerapun, Taninnit
Arpornchayanon, Olarn
Rojanasthien, Sattaya
Kongtawelert, Prachya
Pothacharoen, Peraphan
Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title_full Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title_fullStr Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title_full_unstemmed Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title_short Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
title_sort hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554561/
https://www.ncbi.nlm.nih.gov/pubmed/23253362
http://dx.doi.org/10.1186/1749-799X-7-40
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