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Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic Escherichia coli (APEC) isolates have been incriminated as...

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Autores principales: Johnson, Timothy J., Danzeisen, Jessica L., Trampel, Darrell, Nolan, Lisa K., Seemann, Torsten, Bager, Ragnhild J., Bojesen, Anders M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554606/
https://www.ncbi.nlm.nih.gov/pubmed/23359626
http://dx.doi.org/10.1371/journal.pone.0054844
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author Johnson, Timothy J.
Danzeisen, Jessica L.
Trampel, Darrell
Nolan, Lisa K.
Seemann, Torsten
Bager, Ragnhild J.
Bojesen, Anders M.
author_facet Johnson, Timothy J.
Danzeisen, Jessica L.
Trampel, Darrell
Nolan, Lisa K.
Seemann, Torsten
Bager, Ragnhild J.
Bojesen, Anders M.
author_sort Johnson, Timothy J.
collection PubMed
description Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic Escherichia coli (APEC) isolates have been incriminated as the causative agent of laying hen peritonitis, Gallibacterium anatis are frequently isolated from peritonitis lesions. Despite recent studies suggesting a role for G. anatis in the pathogenesis of peritonitis, little is known about the organism’s virulence mechanisms, genomic composition and population dynamics. Here, we compared the genome sequences of three G. anatis isolates in an effort to understand its virulence mechanisms and identify novel antigenic traits. A multilocus sequence typing method was also established for G. anatis and used to characterize the genotypic relatedness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates. Genomic comparisons suggest that G. anatis is a highly diverse bacterial species, with some strains possessing previously described and potential virulence factors, but with a core genome containing several antigenic candidates. Multilocus sequence typing effectively distinguished 82 sequence types and several clonal complexes of G. anatis, and some clones seemed to predominate among G. anatis populations from commercial layers in Iowa. Biofilm formation and resistance to antimicrobial agents was also observed in several clades. Overall, the genomic diversity of G. anatis suggests that multiple lineages exist with differing pathogenic potential towards birds.
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spelling pubmed-35546062013-01-28 Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry Johnson, Timothy J. Danzeisen, Jessica L. Trampel, Darrell Nolan, Lisa K. Seemann, Torsten Bager, Ragnhild J. Bojesen, Anders M. PLoS One Research Article Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic Escherichia coli (APEC) isolates have been incriminated as the causative agent of laying hen peritonitis, Gallibacterium anatis are frequently isolated from peritonitis lesions. Despite recent studies suggesting a role for G. anatis in the pathogenesis of peritonitis, little is known about the organism’s virulence mechanisms, genomic composition and population dynamics. Here, we compared the genome sequences of three G. anatis isolates in an effort to understand its virulence mechanisms and identify novel antigenic traits. A multilocus sequence typing method was also established for G. anatis and used to characterize the genotypic relatedness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates. Genomic comparisons suggest that G. anatis is a highly diverse bacterial species, with some strains possessing previously described and potential virulence factors, but with a core genome containing several antigenic candidates. Multilocus sequence typing effectively distinguished 82 sequence types and several clonal complexes of G. anatis, and some clones seemed to predominate among G. anatis populations from commercial layers in Iowa. Biofilm formation and resistance to antimicrobial agents was also observed in several clades. Overall, the genomic diversity of G. anatis suggests that multiple lineages exist with differing pathogenic potential towards birds. Public Library of Science 2013-01-24 /pmc/articles/PMC3554606/ /pubmed/23359626 http://dx.doi.org/10.1371/journal.pone.0054844 Text en © 2013 Johnson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Johnson, Timothy J.
Danzeisen, Jessica L.
Trampel, Darrell
Nolan, Lisa K.
Seemann, Torsten
Bager, Ragnhild J.
Bojesen, Anders M.
Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title_full Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title_fullStr Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title_full_unstemmed Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title_short Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
title_sort genome analysis and phylogenetic relatedness of gallibacterium anatis strains from poultry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554606/
https://www.ncbi.nlm.nih.gov/pubmed/23359626
http://dx.doi.org/10.1371/journal.pone.0054844
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