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The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons
The polarized trafficking of axonal and dendritic components is essential for the development and maintenance of neuronal structure and function. Neuropeptide-containing dense-core (DCVs) vesicles are trafficked in a polarized manner from the cell body to their sites of release; however, the molecul...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554613/ https://www.ncbi.nlm.nih.gov/pubmed/23358451 http://dx.doi.org/10.1371/journal.pone.0054763 |
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author | Goodwin, Patricia R. Juo, Peter |
author_facet | Goodwin, Patricia R. Juo, Peter |
author_sort | Goodwin, Patricia R. |
collection | PubMed |
description | The polarized trafficking of axonal and dendritic components is essential for the development and maintenance of neuronal structure and function. Neuropeptide-containing dense-core (DCVs) vesicles are trafficked in a polarized manner from the cell body to their sites of release; however, the molecules involved in this process are not well defined. Here we show that the scaffolding protein SYD-2/Liprin-α is required for the normal polarized localization of Venus-tagged neuropeptides to axons of cholinergic motor neurons in C. elegans. In syd-2 loss of function mutants, the normal polarized localization of INS-22 neuropeptide-containing DCVs in motor neurons is disrupted, and DCVs accumulate in the cell body and dendrites. Time-lapse microscopy and kymograph analysis of mobile DCVs revealed that syd-2 mutants exhibit decreased numbers of DCVs moving in both anterograde and retrograde directions, and a corresponding increase in stationary DCVs in both axon commissures and dendrites. In addition, DCV run lengths and velocities were decreased in both axon commissures and dendrites of syd-2 mutants. This study shows that SYD-2 promotes bi-directional mobility of DCVs and identifies SYD-2 as a novel regulator of DCV trafficking and polarized distribution. |
format | Online Article Text |
id | pubmed-3554613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35546132013-01-28 The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons Goodwin, Patricia R. Juo, Peter PLoS One Research Article The polarized trafficking of axonal and dendritic components is essential for the development and maintenance of neuronal structure and function. Neuropeptide-containing dense-core (DCVs) vesicles are trafficked in a polarized manner from the cell body to their sites of release; however, the molecules involved in this process are not well defined. Here we show that the scaffolding protein SYD-2/Liprin-α is required for the normal polarized localization of Venus-tagged neuropeptides to axons of cholinergic motor neurons in C. elegans. In syd-2 loss of function mutants, the normal polarized localization of INS-22 neuropeptide-containing DCVs in motor neurons is disrupted, and DCVs accumulate in the cell body and dendrites. Time-lapse microscopy and kymograph analysis of mobile DCVs revealed that syd-2 mutants exhibit decreased numbers of DCVs moving in both anterograde and retrograde directions, and a corresponding increase in stationary DCVs in both axon commissures and dendrites. In addition, DCV run lengths and velocities were decreased in both axon commissures and dendrites of syd-2 mutants. This study shows that SYD-2 promotes bi-directional mobility of DCVs and identifies SYD-2 as a novel regulator of DCV trafficking and polarized distribution. Public Library of Science 2013-01-24 /pmc/articles/PMC3554613/ /pubmed/23358451 http://dx.doi.org/10.1371/journal.pone.0054763 Text en © 2013 Goodwin, Juo http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Goodwin, Patricia R. Juo, Peter The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title | The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title_full | The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title_fullStr | The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title_full_unstemmed | The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title_short | The Scaffolding Protein SYD-2/Liprin-α Regulates the Mobility and Polarized Distribution of Dense-Core Vesicles in C. elegans Motor Neurons |
title_sort | scaffolding protein syd-2/liprin-α regulates the mobility and polarized distribution of dense-core vesicles in c. elegans motor neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554613/ https://www.ncbi.nlm.nih.gov/pubmed/23358451 http://dx.doi.org/10.1371/journal.pone.0054763 |
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