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Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment

Gastric cancer is associated with chronic inflammation and Helicobacter pylori infection. Th17 cells are CD4(+) T cells associated with infections and inflammation; but their role and mechanism of induction during carcinogenesis is not understood. Gastric myofibroblasts/fibroblasts (GMF) are abundan...

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Autores principales: Pinchuk, Irina V., Morris, Katherine T., Nofchissey, Robert A., Earley, Rachel B., Wu, Jeng-Yih, Ma, Thomas Y., Beswick, Ellen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554710/
https://www.ncbi.nlm.nih.gov/pubmed/23365642
http://dx.doi.org/10.1371/journal.pone.0053798
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author Pinchuk, Irina V.
Morris, Katherine T.
Nofchissey, Robert A.
Earley, Rachel B.
Wu, Jeng-Yih
Ma, Thomas Y.
Beswick, Ellen J.
author_facet Pinchuk, Irina V.
Morris, Katherine T.
Nofchissey, Robert A.
Earley, Rachel B.
Wu, Jeng-Yih
Ma, Thomas Y.
Beswick, Ellen J.
author_sort Pinchuk, Irina V.
collection PubMed
description Gastric cancer is associated with chronic inflammation and Helicobacter pylori infection. Th17 cells are CD4(+) T cells associated with infections and inflammation; but their role and mechanism of induction during carcinogenesis is not understood. Gastric myofibroblasts/fibroblasts (GMF) are abundant class II MHC expressing cells that act as novel antigen presenting cells. Here we have demonstrated the accumulation of Th17 in H. pylori-infected human tissues and in the gastric tumor microenvironment. GMF isolated from human gastric cancer and H. pylori infected tissues co-cultured with CD4(+) T cells induced substantially higher levels of Th17 than GMF from normal tissues in an IL-6, TGF-β, and IL-21 dependent manner. Th17 required interaction with class II MHC on GMF for activation and proliferation. These studies suggest that Th17 are induced during both H. pylori infection and gastric cancer in the inflammatory milieu of gastric stroma and may be an important link between inflammation and carcinogenesis.
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spelling pubmed-35547102013-01-30 Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment Pinchuk, Irina V. Morris, Katherine T. Nofchissey, Robert A. Earley, Rachel B. Wu, Jeng-Yih Ma, Thomas Y. Beswick, Ellen J. PLoS One Research Article Gastric cancer is associated with chronic inflammation and Helicobacter pylori infection. Th17 cells are CD4(+) T cells associated with infections and inflammation; but their role and mechanism of induction during carcinogenesis is not understood. Gastric myofibroblasts/fibroblasts (GMF) are abundant class II MHC expressing cells that act as novel antigen presenting cells. Here we have demonstrated the accumulation of Th17 in H. pylori-infected human tissues and in the gastric tumor microenvironment. GMF isolated from human gastric cancer and H. pylori infected tissues co-cultured with CD4(+) T cells induced substantially higher levels of Th17 than GMF from normal tissues in an IL-6, TGF-β, and IL-21 dependent manner. Th17 required interaction with class II MHC on GMF for activation and proliferation. These studies suggest that Th17 are induced during both H. pylori infection and gastric cancer in the inflammatory milieu of gastric stroma and may be an important link between inflammation and carcinogenesis. Public Library of Science 2013-01-24 /pmc/articles/PMC3554710/ /pubmed/23365642 http://dx.doi.org/10.1371/journal.pone.0053798 Text en © 2013 Pinchuk et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pinchuk, Irina V.
Morris, Katherine T.
Nofchissey, Robert A.
Earley, Rachel B.
Wu, Jeng-Yih
Ma, Thomas Y.
Beswick, Ellen J.
Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title_full Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title_fullStr Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title_full_unstemmed Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title_short Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment
title_sort stromal cells induce th17 during helicobacter pylori infection and in the gastric tumor microenvironment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554710/
https://www.ncbi.nlm.nih.gov/pubmed/23365642
http://dx.doi.org/10.1371/journal.pone.0053798
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