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Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress

Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. T...

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Autores principales: Li, Mingfeng, Sun, Hongxing, Feng, Qiong, Lu, Huiming, Zhao, Ye, Zhang, Hui, Xu, Xin, Jiao, Jiandong, Wang, Liangyan, Hua, Yuejin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554781/
https://www.ncbi.nlm.nih.gov/pubmed/23365666
http://dx.doi.org/10.1371/journal.pone.0054420
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author Li, Mingfeng
Sun, Hongxing
Feng, Qiong
Lu, Huiming
Zhao, Ye
Zhang, Hui
Xu, Xin
Jiao, Jiandong
Wang, Liangyan
Hua, Yuejin
author_facet Li, Mingfeng
Sun, Hongxing
Feng, Qiong
Lu, Huiming
Zhao, Ye
Zhang, Hui
Xu, Xin
Jiao, Jiandong
Wang, Liangyan
Hua, Yuejin
author_sort Li, Mingfeng
collection PubMed
description Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. The results showed that the extracellular DNA fragments inhibited the growth of D. radiodurans. Interestingly, dGMP, a DNA component, enhanced D. radiodurans tolerance to H(2)O(2) and gamma-radiation significantly. Further experiments indicated that extracellular dGMP stimulated the activity of one catalase (KatA, DR1998), and induced gene transcription including the extracellular nuclease (drb0067). When this only extracellular nuclease gene (drb0067) in D. radiodurans was deleted, the mutant strain showed more sensitive to H(2)O(2) and gamma-radiation than the wild type strain. These results suggest that DRB0067 plays an important role in oxidative stress resistance. Taken together, we proposed a new anti-oxidation mechanism in D. radiodurans. This mechanism acts to increase expression levels of DRB0067 which then secretes active nuclease to degrade extracellular DNA fragments. The extracellular nuclease has a two-fold benefit, creating more free dNTPs for further cell protection and the removal of extracellular DNA fragments.
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spelling pubmed-35547812013-01-30 Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress Li, Mingfeng Sun, Hongxing Feng, Qiong Lu, Huiming Zhao, Ye Zhang, Hui Xu, Xin Jiao, Jiandong Wang, Liangyan Hua, Yuejin PLoS One Research Article Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. The results showed that the extracellular DNA fragments inhibited the growth of D. radiodurans. Interestingly, dGMP, a DNA component, enhanced D. radiodurans tolerance to H(2)O(2) and gamma-radiation significantly. Further experiments indicated that extracellular dGMP stimulated the activity of one catalase (KatA, DR1998), and induced gene transcription including the extracellular nuclease (drb0067). When this only extracellular nuclease gene (drb0067) in D. radiodurans was deleted, the mutant strain showed more sensitive to H(2)O(2) and gamma-radiation than the wild type strain. These results suggest that DRB0067 plays an important role in oxidative stress resistance. Taken together, we proposed a new anti-oxidation mechanism in D. radiodurans. This mechanism acts to increase expression levels of DRB0067 which then secretes active nuclease to degrade extracellular DNA fragments. The extracellular nuclease has a two-fold benefit, creating more free dNTPs for further cell protection and the removal of extracellular DNA fragments. Public Library of Science 2013-01-24 /pmc/articles/PMC3554781/ /pubmed/23365666 http://dx.doi.org/10.1371/journal.pone.0054420 Text en © 2013 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Mingfeng
Sun, Hongxing
Feng, Qiong
Lu, Huiming
Zhao, Ye
Zhang, Hui
Xu, Xin
Jiao, Jiandong
Wang, Liangyan
Hua, Yuejin
Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title_full Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title_fullStr Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title_full_unstemmed Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title_short Extracellular dGMP Enhances Deinococcus radiodurans Tolerance to Oxidative Stress
title_sort extracellular dgmp enhances deinococcus radiodurans tolerance to oxidative stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554781/
https://www.ncbi.nlm.nih.gov/pubmed/23365666
http://dx.doi.org/10.1371/journal.pone.0054420
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