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Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis

Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosom...

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Detalles Bibliográficos
Autores principales: Egami, Youhei, Araki, Nobukazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554782/
https://www.ncbi.nlm.nih.gov/pubmed/23378675
http://dx.doi.org/10.1267/ahc.12014
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author Egami, Youhei
Araki, Nobukazu
author_facet Egami, Youhei
Araki, Nobukazu
author_sort Egami, Youhei
collection PubMed
description Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosomal membranes. During the early stage of macropinosome formation, Rab20 was slightly localized on the circular ruffles (macropinocytic cups), the precursor forms of macropinosomes, and was increasingly recruited to the newly formed macropinosomes. Although Rab20 was colocalized with Rab5 and Rab21 on macropinosomal membranes, the association of Rab20 with macropinosomes persisted even after the dissociations of Rab5 and Rab21 from macropinosomal membranes. Rab20 was then colocalized with Rab7 and Lamp1, late endosomal/lysosomal markers, on macropinosomes for a while. Our data indicate that Rab20 is a novel component of macropinocytic pathway and functions at long-standing stages from early to late macropinosome maturation.
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spelling pubmed-35547822013-02-01 Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis Egami, Youhei Araki, Nobukazu Acta Histochem Cytochem Regular Article Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosomal membranes. During the early stage of macropinosome formation, Rab20 was slightly localized on the circular ruffles (macropinocytic cups), the precursor forms of macropinosomes, and was increasingly recruited to the newly formed macropinosomes. Although Rab20 was colocalized with Rab5 and Rab21 on macropinosomal membranes, the association of Rab20 with macropinosomes persisted even after the dissociations of Rab5 and Rab21 from macropinosomal membranes. Rab20 was then colocalized with Rab7 and Lamp1, late endosomal/lysosomal markers, on macropinosomes for a while. Our data indicate that Rab20 is a novel component of macropinocytic pathway and functions at long-standing stages from early to late macropinosome maturation. Japan Society of Histochemistry and Cytochemistry 2012-12-26 2012-11-03 /pmc/articles/PMC3554782/ /pubmed/23378675 http://dx.doi.org/10.1267/ahc.12014 Text en © 2012 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Egami, Youhei
Araki, Nobukazu
Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title_full Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title_fullStr Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title_full_unstemmed Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title_short Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
title_sort spatiotemporal localization of rab20 in live raw264 macrophages during macropinocytosis
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554782/
https://www.ncbi.nlm.nih.gov/pubmed/23378675
http://dx.doi.org/10.1267/ahc.12014
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