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Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis
Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosom...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Japan Society of Histochemistry and Cytochemistry
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554782/ https://www.ncbi.nlm.nih.gov/pubmed/23378675 http://dx.doi.org/10.1267/ahc.12014 |
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author | Egami, Youhei Araki, Nobukazu |
author_facet | Egami, Youhei Araki, Nobukazu |
author_sort | Egami, Youhei |
collection | PubMed |
description | Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosomal membranes. During the early stage of macropinosome formation, Rab20 was slightly localized on the circular ruffles (macropinocytic cups), the precursor forms of macropinosomes, and was increasingly recruited to the newly formed macropinosomes. Although Rab20 was colocalized with Rab5 and Rab21 on macropinosomal membranes, the association of Rab20 with macropinosomes persisted even after the dissociations of Rab5 and Rab21 from macropinosomal membranes. Rab20 was then colocalized with Rab7 and Lamp1, late endosomal/lysosomal markers, on macropinosomes for a while. Our data indicate that Rab20 is a novel component of macropinocytic pathway and functions at long-standing stages from early to late macropinosome maturation. |
format | Online Article Text |
id | pubmed-3554782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Japan Society of Histochemistry and Cytochemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-35547822013-02-01 Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis Egami, Youhei Araki, Nobukazu Acta Histochem Cytochem Regular Article Rab20 is a member of the Rab GTPase family, but its implication in macropinocytosis is unclear. We examined the spatiotemporal localization of Rab20 in RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab20. It was shown that Rab20 was transiently associated with macropinosomal membranes. During the early stage of macropinosome formation, Rab20 was slightly localized on the circular ruffles (macropinocytic cups), the precursor forms of macropinosomes, and was increasingly recruited to the newly formed macropinosomes. Although Rab20 was colocalized with Rab5 and Rab21 on macropinosomal membranes, the association of Rab20 with macropinosomes persisted even after the dissociations of Rab5 and Rab21 from macropinosomal membranes. Rab20 was then colocalized with Rab7 and Lamp1, late endosomal/lysosomal markers, on macropinosomes for a while. Our data indicate that Rab20 is a novel component of macropinocytic pathway and functions at long-standing stages from early to late macropinosome maturation. Japan Society of Histochemistry and Cytochemistry 2012-12-26 2012-11-03 /pmc/articles/PMC3554782/ /pubmed/23378675 http://dx.doi.org/10.1267/ahc.12014 Text en © 2012 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Egami, Youhei Araki, Nobukazu Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title | Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title_full | Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title_fullStr | Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title_full_unstemmed | Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title_short | Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis |
title_sort | spatiotemporal localization of rab20 in live raw264 macrophages during macropinocytosis |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554782/ https://www.ncbi.nlm.nih.gov/pubmed/23378675 http://dx.doi.org/10.1267/ahc.12014 |
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