Agouti C57BL/6N embryonic stem cells for mouse genetic resources

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant Agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for la...

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Detalles Bibliográficos
Autores principales: Pettitt, Stephen J., Liang, Qi, Rairdan, Xin Y., Moran, Jennifer L., Prosser, Haydn M., Beier, David R., Lloyd, Kent, Bradley, Allan, Skarnes, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555078/
https://www.ncbi.nlm.nih.gov/pubmed/19525957
http://dx.doi.org/10.1038/nmeth.1342
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author Pettitt, Stephen J.
Liang, Qi
Rairdan, Xin Y.
Moran, Jennifer L.
Prosser, Haydn M.
Beier, David R.
Lloyd, Kent
Bradley, Allan
Skarnes, William C.
author_facet Pettitt, Stephen J.
Liang, Qi
Rairdan, Xin Y.
Moran, Jennifer L.
Prosser, Haydn M.
Beier, David R.
Lloyd, Kent
Bradley, Allan
Skarnes, William C.
author_sort Pettitt, Stephen J.
collection PubMed
description We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant Agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
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spelling pubmed-35550782013-01-25 Agouti C57BL/6N embryonic stem cells for mouse genetic resources Pettitt, Stephen J. Liang, Qi Rairdan, Xin Y. Moran, Jennifer L. Prosser, Haydn M. Beier, David R. Lloyd, Kent Bradley, Allan Skarnes, William C. Nat Methods Article We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant Agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background. 2009-06-14 2009-07 /pmc/articles/PMC3555078/ /pubmed/19525957 http://dx.doi.org/10.1038/nmeth.1342 Text en
spellingShingle Article
Pettitt, Stephen J.
Liang, Qi
Rairdan, Xin Y.
Moran, Jennifer L.
Prosser, Haydn M.
Beier, David R.
Lloyd, Kent
Bradley, Allan
Skarnes, William C.
Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title_full Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title_fullStr Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title_full_unstemmed Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title_short Agouti C57BL/6N embryonic stem cells for mouse genetic resources
title_sort agouti c57bl/6n embryonic stem cells for mouse genetic resources
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555078/
https://www.ncbi.nlm.nih.gov/pubmed/19525957
http://dx.doi.org/10.1038/nmeth.1342
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