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miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds

microRNAs (miRNAs) are important post-transcriptional regulators in eukaryotes that target mRNAs repressing their expression. The uncertain process of pig domestication, with different origin focuses, and the selection process that commercial breeds suffered, have generated a wide spectrum of breeds...

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Autores principales: Timoneda, Oriol, Balcells, Ingrid, Núñez, Jose Ignacio, Egea, Raquel, Vera, Gonzalo, Castelló, Anna, Tomàs, Anna, Sánchez, Armand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555835/
https://www.ncbi.nlm.nih.gov/pubmed/23372853
http://dx.doi.org/10.1371/journal.pone.0055402
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author Timoneda, Oriol
Balcells, Ingrid
Núñez, Jose Ignacio
Egea, Raquel
Vera, Gonzalo
Castelló, Anna
Tomàs, Anna
Sánchez, Armand
author_facet Timoneda, Oriol
Balcells, Ingrid
Núñez, Jose Ignacio
Egea, Raquel
Vera, Gonzalo
Castelló, Anna
Tomàs, Anna
Sánchez, Armand
author_sort Timoneda, Oriol
collection PubMed
description microRNAs (miRNAs) are important post-transcriptional regulators in eukaryotes that target mRNAs repressing their expression. The uncertain process of pig domestication, with different origin focuses, and the selection process that commercial breeds suffered, have generated a wide spectrum of breeds with clear genetic and phenotypic variability. The aim of this work was to define the miRNAs expression profile in kidney of several porcine breeds. Small RNA libraries from kidney were elaborated and high-throughput sequenced with the 454 Genome Sequencer FLX (Roche). Pigs used were classified into three groups: the European origin group (Iberian breed and European Wild Boar ancestor), European commercial breeds (Landrace, Large White and Piétrain breeds) and breeds with Asian origin (Meishan and Vietnamese breeds). A total of 229 miRNAs were described in the pig kidney miRNA profile, including 110 miRNAs out of the 257 previously described pig miRNAs and 119 orthologous miRNAs. The most expressed miRNAs in pig kidney microRNAome were Hsa-miR-200b-3p, Ssc-miR-125b and Ssc-miR-23b. Moreover, 5 novel porcine miRNAs and 3 orthologous miRNAs could be validated through RT-qPCR. miRNA sequence variation was determined in 116 miRNAs, evidencing the presence of isomiRs. 125 miRNAs were differentially expressed between breed groups. The identification of breed-specific miRNAs, which could be potentially associated to certain phenotypes, is becoming a new tool for the study of the genetic variability underlying complex traits and furthermore, it adds a new layer of complexity to the interesting process of pig evolution.
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spelling pubmed-35558352013-01-31 miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds Timoneda, Oriol Balcells, Ingrid Núñez, Jose Ignacio Egea, Raquel Vera, Gonzalo Castelló, Anna Tomàs, Anna Sánchez, Armand PLoS One Research Article microRNAs (miRNAs) are important post-transcriptional regulators in eukaryotes that target mRNAs repressing their expression. The uncertain process of pig domestication, with different origin focuses, and the selection process that commercial breeds suffered, have generated a wide spectrum of breeds with clear genetic and phenotypic variability. The aim of this work was to define the miRNAs expression profile in kidney of several porcine breeds. Small RNA libraries from kidney were elaborated and high-throughput sequenced with the 454 Genome Sequencer FLX (Roche). Pigs used were classified into three groups: the European origin group (Iberian breed and European Wild Boar ancestor), European commercial breeds (Landrace, Large White and Piétrain breeds) and breeds with Asian origin (Meishan and Vietnamese breeds). A total of 229 miRNAs were described in the pig kidney miRNA profile, including 110 miRNAs out of the 257 previously described pig miRNAs and 119 orthologous miRNAs. The most expressed miRNAs in pig kidney microRNAome were Hsa-miR-200b-3p, Ssc-miR-125b and Ssc-miR-23b. Moreover, 5 novel porcine miRNAs and 3 orthologous miRNAs could be validated through RT-qPCR. miRNA sequence variation was determined in 116 miRNAs, evidencing the presence of isomiRs. 125 miRNAs were differentially expressed between breed groups. The identification of breed-specific miRNAs, which could be potentially associated to certain phenotypes, is becoming a new tool for the study of the genetic variability underlying complex traits and furthermore, it adds a new layer of complexity to the interesting process of pig evolution. Public Library of Science 2013-01-25 /pmc/articles/PMC3555835/ /pubmed/23372853 http://dx.doi.org/10.1371/journal.pone.0055402 Text en © 2013 Timoneda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Timoneda, Oriol
Balcells, Ingrid
Núñez, Jose Ignacio
Egea, Raquel
Vera, Gonzalo
Castelló, Anna
Tomàs, Anna
Sánchez, Armand
miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title_full miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title_fullStr miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title_full_unstemmed miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title_short miRNA Expression Profile Analysis in Kidney of Different Porcine Breeds
title_sort mirna expression profile analysis in kidney of different porcine breeds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555835/
https://www.ncbi.nlm.nih.gov/pubmed/23372853
http://dx.doi.org/10.1371/journal.pone.0055402
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