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Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells

BACKGROUND AND THE PURPOSE OF THE STUDY: Natural products from plants have an important role in the development and production of new drugs mainly for cancer therapy. More recently, we have shown that the pericarp methanolic extract of Pistacia atlantica sub kurdica (with local name of Baneh) as a r...

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Autores principales: Rezaei, Parisa Fathi, Fouladdel, Shamileh, Ghaffari, Seyed Mahmood, Amin, Gholamreza, Azizi, Ebrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556048/
https://www.ncbi.nlm.nih.gov/pubmed/23351343
http://dx.doi.org/10.1186/2008-2231-20-101
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author Rezaei, Parisa Fathi
Fouladdel, Shamileh
Ghaffari, Seyed Mahmood
Amin, Gholamreza
Azizi, Ebrahim
author_facet Rezaei, Parisa Fathi
Fouladdel, Shamileh
Ghaffari, Seyed Mahmood
Amin, Gholamreza
Azizi, Ebrahim
author_sort Rezaei, Parisa Fathi
collection PubMed
description BACKGROUND AND THE PURPOSE OF THE STUDY: Natural products from plants have an important role in the development and production of new drugs mainly for cancer therapy. More recently, we have shown that the pericarp methanolic extract of Pistacia atlantica sub kurdica (with local name of Baneh) as a rich source of active biological components with high antioxidant and radical scavenging activities, has ability to cease proliferation and induce apoptosis in T47D human breast cancer cells. The present study aimed to clarify whether Baneh extract able to alter cell cycle progression of T47D cells or not. METHODS: In order to study the possible effect of Baneh extract on cell cycle of T47D cells, we evaluated cell cycle distribution and its regulatory proteins by flow cytometry and western blot analysis respectively. RESULTS: Baneh extract induced G(0)/G(1) cell cycle arrest in conjunction with a marked decrease in expression of cyclin D1 and cdk4 that was strongly dependent on time of exposure. In parallel, Dox-treated T47D cells in early time points were accumulated on S phase, but after 48 h cell cycle progression was inhibited on G(2)/M. Dox promoted striking accumulation of cyclin B1 rapidly and enhanced cyclin A abundance. CONCLUSION: Taken together, our results establish that the antitumor activity of the pericarp extract of Baneh partly is mediated via cell cycle arrest and downregulation of cyclin D1 and cdk4 expression. These findings warrant further evaluation regarding the mechanism(s) of action of this promising anticancer agent.
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spelling pubmed-35560482013-01-31 Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells Rezaei, Parisa Fathi Fouladdel, Shamileh Ghaffari, Seyed Mahmood Amin, Gholamreza Azizi, Ebrahim Daru Research Article BACKGROUND AND THE PURPOSE OF THE STUDY: Natural products from plants have an important role in the development and production of new drugs mainly for cancer therapy. More recently, we have shown that the pericarp methanolic extract of Pistacia atlantica sub kurdica (with local name of Baneh) as a rich source of active biological components with high antioxidant and radical scavenging activities, has ability to cease proliferation and induce apoptosis in T47D human breast cancer cells. The present study aimed to clarify whether Baneh extract able to alter cell cycle progression of T47D cells or not. METHODS: In order to study the possible effect of Baneh extract on cell cycle of T47D cells, we evaluated cell cycle distribution and its regulatory proteins by flow cytometry and western blot analysis respectively. RESULTS: Baneh extract induced G(0)/G(1) cell cycle arrest in conjunction with a marked decrease in expression of cyclin D1 and cdk4 that was strongly dependent on time of exposure. In parallel, Dox-treated T47D cells in early time points were accumulated on S phase, but after 48 h cell cycle progression was inhibited on G(2)/M. Dox promoted striking accumulation of cyclin B1 rapidly and enhanced cyclin A abundance. CONCLUSION: Taken together, our results establish that the antitumor activity of the pericarp extract of Baneh partly is mediated via cell cycle arrest and downregulation of cyclin D1 and cdk4 expression. These findings warrant further evaluation regarding the mechanism(s) of action of this promising anticancer agent. BioMed Central 2012-12-28 /pmc/articles/PMC3556048/ /pubmed/23351343 http://dx.doi.org/10.1186/2008-2231-20-101 Text en Copyright ©2012 Rezaei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rezaei, Parisa Fathi
Fouladdel, Shamileh
Ghaffari, Seyed Mahmood
Amin, Gholamreza
Azizi, Ebrahim
Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title_full Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title_fullStr Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title_full_unstemmed Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title_short Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells
title_sort induction of g1 cell cycle arrest and cyclin d1 down-regulation in response to pericarp extract of baneh in human breast cancer t47d cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556048/
https://www.ncbi.nlm.nih.gov/pubmed/23351343
http://dx.doi.org/10.1186/2008-2231-20-101
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