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Seroprevalence of porcine torovirus (PToV) in Spanish farms
BACKGROUND: Torovirus infections have been associated with gastroenteritis and diarrhea in horses, cows, pigs and humans, especially in young animals and in children. Although asymptomatic in a large percentage of cases, however toroviruses may pose a potential threat to worsen disease outcome in co...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556161/ https://www.ncbi.nlm.nih.gov/pubmed/23217216 http://dx.doi.org/10.1186/1756-0500-5-675 |
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author | Alonso-Padilla, Julio Pignatelli, Jaime Simon-Grifé, Meritxell Plazuelo, Susana Casal, Jordi Rodríguez, Dolores |
author_facet | Alonso-Padilla, Julio Pignatelli, Jaime Simon-Grifé, Meritxell Plazuelo, Susana Casal, Jordi Rodríguez, Dolores |
author_sort | Alonso-Padilla, Julio |
collection | PubMed |
description | BACKGROUND: Torovirus infections have been associated with gastroenteritis and diarrhea in horses, cows, pigs and humans, especially in young animals and in children. Although asymptomatic in a large percentage of cases, however toroviruses may pose a potential threat to worsen disease outcome in concurrent infections with other enteric pathogens. Previous studies based on the analysis of limited numbers of samples indicated high seroprevalences against porcine torovirus (PToV) in various European countries. The aim of this work was to perform a seroepidemiological survey of PToV in Spanish farms in order to define the seroprevalence against this virus. RESULTS: Serum samples (n = 2664) from pigs of different ages were collected from 100 Spanish farms coming from 10 regions that concentrate 96.1% of the 3392 farms with 80 or more sows censused in Spain. Samples were screened by means of an indirect enzyme-linked immune-sorbent assay (ELISA) based on a recombinant PToV nucleocapsid protein as antigen. The analysis of the whole serum collection yielded a total of 95.7% (2550/2664) seropositive samples. The highest prevalence (99.6%, 1382/1388) and ELISA values (average O.D. ± standard deviation) were observed in the sows (1.03±0.36) and the lowest prevalence (59.4%, 98/165) and anti-PToV IgG levels (0.45±0.16) were found amongst 3-week-old piglets. Both ELISA reactivity values and seroprevalence percentages rose quickly with piglet’s age from 3 to 11 weeks of age; the seroprevalence was 99.3% (2254/2270) when only the samples from sows and pigs over 11-weeks of age were considered. Antibodies against PToV were detected in all analyzed farms. CONCLUSIONS: This report describes the results of the largest torovirus seroepidemiological survey in farmed swine performed so far. Overall, the seroprevalence against PToV in animals older than 11 weeks of age was >99%, indicating that this virus is endemic in pig herds from Spain. |
format | Online Article Text |
id | pubmed-3556161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35561612013-01-31 Seroprevalence of porcine torovirus (PToV) in Spanish farms Alonso-Padilla, Julio Pignatelli, Jaime Simon-Grifé, Meritxell Plazuelo, Susana Casal, Jordi Rodríguez, Dolores BMC Res Notes Research Article BACKGROUND: Torovirus infections have been associated with gastroenteritis and diarrhea in horses, cows, pigs and humans, especially in young animals and in children. Although asymptomatic in a large percentage of cases, however toroviruses may pose a potential threat to worsen disease outcome in concurrent infections with other enteric pathogens. Previous studies based on the analysis of limited numbers of samples indicated high seroprevalences against porcine torovirus (PToV) in various European countries. The aim of this work was to perform a seroepidemiological survey of PToV in Spanish farms in order to define the seroprevalence against this virus. RESULTS: Serum samples (n = 2664) from pigs of different ages were collected from 100 Spanish farms coming from 10 regions that concentrate 96.1% of the 3392 farms with 80 or more sows censused in Spain. Samples were screened by means of an indirect enzyme-linked immune-sorbent assay (ELISA) based on a recombinant PToV nucleocapsid protein as antigen. The analysis of the whole serum collection yielded a total of 95.7% (2550/2664) seropositive samples. The highest prevalence (99.6%, 1382/1388) and ELISA values (average O.D. ± standard deviation) were observed in the sows (1.03±0.36) and the lowest prevalence (59.4%, 98/165) and anti-PToV IgG levels (0.45±0.16) were found amongst 3-week-old piglets. Both ELISA reactivity values and seroprevalence percentages rose quickly with piglet’s age from 3 to 11 weeks of age; the seroprevalence was 99.3% (2254/2270) when only the samples from sows and pigs over 11-weeks of age were considered. Antibodies against PToV were detected in all analyzed farms. CONCLUSIONS: This report describes the results of the largest torovirus seroepidemiological survey in farmed swine performed so far. Overall, the seroprevalence against PToV in animals older than 11 weeks of age was >99%, indicating that this virus is endemic in pig herds from Spain. BioMed Central 2012-12-05 /pmc/articles/PMC3556161/ /pubmed/23217216 http://dx.doi.org/10.1186/1756-0500-5-675 Text en Copyright ©2012 Alonso-Padilla et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Alonso-Padilla, Julio Pignatelli, Jaime Simon-Grifé, Meritxell Plazuelo, Susana Casal, Jordi Rodríguez, Dolores Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title | Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title_full | Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title_fullStr | Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title_full_unstemmed | Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title_short | Seroprevalence of porcine torovirus (PToV) in Spanish farms |
title_sort | seroprevalence of porcine torovirus (ptov) in spanish farms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556161/ https://www.ncbi.nlm.nih.gov/pubmed/23217216 http://dx.doi.org/10.1186/1756-0500-5-675 |
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