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Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo

BACKGROUND: Gleditsia sinensis thorns have been widely used in traditional Korean medicine for the treatment of several diseases, including obesity, thrombosis, and tumor-related diseases. The aim of the study is to determine the antiangiogenic effect of Gleditsia sinensis thorns in vitro and in viv...

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Autores principales: Yi, Jin-Mu, Park, Jong-Shik, Oh, Se-Mi, Lee, Jun, Kim, Jinhee, Oh, Dal-Seok, Bang, Ok-Sun, Kim, No Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556500/
https://www.ncbi.nlm.nih.gov/pubmed/23206527
http://dx.doi.org/10.1186/1472-6882-12-243
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author Yi, Jin-Mu
Park, Jong-Shik
Oh, Se-Mi
Lee, Jun
Kim, Jinhee
Oh, Dal-Seok
Bang, Ok-Sun
Kim, No Soo
author_facet Yi, Jin-Mu
Park, Jong-Shik
Oh, Se-Mi
Lee, Jun
Kim, Jinhee
Oh, Dal-Seok
Bang, Ok-Sun
Kim, No Soo
author_sort Yi, Jin-Mu
collection PubMed
description BACKGROUND: Gleditsia sinensis thorns have been widely used in traditional Korean medicine for the treatment of several diseases, including obesity, thrombosis, and tumor-related diseases. The aim of the study is to determine the antiangiogenic effect of Gleditsia sinensis thorns in vitro and in vivo in a bid to evaluate its potential as an anticancer drug. METHODS: Ethanol extract of Gleditsia sinensis thorns (EEGS) were prepared and used for in vitro and in vivo assays. In vitro antiangiogenic effect of EEGS was determined in HUVEC primary cells by cell migration and tube formation assays. In vivo antiangiogenic effect of EEGS was determined by measuring vessel formation and vascular endothelial cells migrating into the implanted matrigels in nude mice. The angiogenesis-related proteins of which expression levels were altered by EEGS were identified by proteomic analysis. RESULTS: EEGS exerted a dose-dependent antiproliferative effect on HUVEC cells without significant cytotoxicity. Angiogenic properties, such as cell migration and tube formation, were significantly inhibited by EEGS in a dose-dependent manner. New vessel formation was also suppressed by EEGS, as determined by the directed in vivo angiogenesis assays in nude mice. EEGS reduced the expression of proangiogenic proteins, endothelin 1 and matrix metallopeptidase 2, in HUVEC cells. CONCLUSIONS: Our findings suggest that EEGS can inhibit angiogenesis by down-regulating proangiogenic proteins, and therefore it should be considered as a potential anticancer drug targeting tumor-derived angiogenesis.
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spelling pubmed-35565002013-01-29 Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo Yi, Jin-Mu Park, Jong-Shik Oh, Se-Mi Lee, Jun Kim, Jinhee Oh, Dal-Seok Bang, Ok-Sun Kim, No Soo BMC Complement Altern Med Research Article BACKGROUND: Gleditsia sinensis thorns have been widely used in traditional Korean medicine for the treatment of several diseases, including obesity, thrombosis, and tumor-related diseases. The aim of the study is to determine the antiangiogenic effect of Gleditsia sinensis thorns in vitro and in vivo in a bid to evaluate its potential as an anticancer drug. METHODS: Ethanol extract of Gleditsia sinensis thorns (EEGS) were prepared and used for in vitro and in vivo assays. In vitro antiangiogenic effect of EEGS was determined in HUVEC primary cells by cell migration and tube formation assays. In vivo antiangiogenic effect of EEGS was determined by measuring vessel formation and vascular endothelial cells migrating into the implanted matrigels in nude mice. The angiogenesis-related proteins of which expression levels were altered by EEGS were identified by proteomic analysis. RESULTS: EEGS exerted a dose-dependent antiproliferative effect on HUVEC cells without significant cytotoxicity. Angiogenic properties, such as cell migration and tube formation, were significantly inhibited by EEGS in a dose-dependent manner. New vessel formation was also suppressed by EEGS, as determined by the directed in vivo angiogenesis assays in nude mice. EEGS reduced the expression of proangiogenic proteins, endothelin 1 and matrix metallopeptidase 2, in HUVEC cells. CONCLUSIONS: Our findings suggest that EEGS can inhibit angiogenesis by down-regulating proangiogenic proteins, and therefore it should be considered as a potential anticancer drug targeting tumor-derived angiogenesis. BioMed Central 2012-12-04 /pmc/articles/PMC3556500/ /pubmed/23206527 http://dx.doi.org/10.1186/1472-6882-12-243 Text en Copyright ©2012 Yi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yi, Jin-Mu
Park, Jong-Shik
Oh, Se-Mi
Lee, Jun
Kim, Jinhee
Oh, Dal-Seok
Bang, Ok-Sun
Kim, No Soo
Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title_full Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title_fullStr Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title_full_unstemmed Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title_short Ethanol extract of Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
title_sort ethanol extract of gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556500/
https://www.ncbi.nlm.nih.gov/pubmed/23206527
http://dx.doi.org/10.1186/1472-6882-12-243
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