Cargando…

Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling

Special attention has recently been drawn to the molecular network of different genes that are responsible for the development of erythroid cells. The aim of the present study was to establish in detail the immunophenotype of early erythroid cells and to compare the gene expression profile of freshl...

Descripción completa

Detalles Bibliográficos
Autores principales: Machherndl-Spandl, S, Suessner, S, Danzer, M, Proell, J, Gabriel, C, Lauf, J, Sylie, R, Klein, H-U, Béné, M C, Weltermann, A, Bettelheim, P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556575/
https://www.ncbi.nlm.nih.gov/pubmed/23310930
http://dx.doi.org/10.1038/bcj.2012.45
_version_ 1782257205638070272
author Machherndl-Spandl, S
Suessner, S
Danzer, M
Proell, J
Gabriel, C
Lauf, J
Sylie, R
Klein, H-U
Béné, M C
Weltermann, A
Bettelheim, P
author_facet Machherndl-Spandl, S
Suessner, S
Danzer, M
Proell, J
Gabriel, C
Lauf, J
Sylie, R
Klein, H-U
Béné, M C
Weltermann, A
Bettelheim, P
author_sort Machherndl-Spandl, S
collection PubMed
description Special attention has recently been drawn to the molecular network of different genes that are responsible for the development of erythroid cells. The aim of the present study was to establish in detail the immunophenotype of early erythroid cells and to compare the gene expression profile of freshly isolated early erythroid precursors with that of the CD34-positive (CD34(+)) compartment. Multiparameter flow cytometric analyses of human bone marrow mononuclear cell fractions (n=20) defined three distinct early erythroid stages. The gene expression profile of sorted early erythroid cells was analyzed by Affymetrix array technology. For 4524 genes, a differential regulation was found in CD105-positive erythroid cells as compared with the CD34(+) progenitor compartment (2362 upregulated genes). A highly significant difference was observed in the expression level of genes involved in transcription, heme synthesis, iron and mitochondrial metabolism and transforming growth factor-β signaling. A comparison with recently published data showed over 1000 genes that as yet have not been reported to be upregulated in the early erythroid lineage. The gene expression level within distinct pathways could be illustrated directly by applying the Ingenuity software program. The results of gene expression analyses can be seen at the Gene Expression Omnibus repository.
format Online
Article
Text
id pubmed-3556575
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-35565752013-01-28 Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling Machherndl-Spandl, S Suessner, S Danzer, M Proell, J Gabriel, C Lauf, J Sylie, R Klein, H-U Béné, M C Weltermann, A Bettelheim, P Blood Cancer J Original Article Special attention has recently been drawn to the molecular network of different genes that are responsible for the development of erythroid cells. The aim of the present study was to establish in detail the immunophenotype of early erythroid cells and to compare the gene expression profile of freshly isolated early erythroid precursors with that of the CD34-positive (CD34(+)) compartment. Multiparameter flow cytometric analyses of human bone marrow mononuclear cell fractions (n=20) defined three distinct early erythroid stages. The gene expression profile of sorted early erythroid cells was analyzed by Affymetrix array technology. For 4524 genes, a differential regulation was found in CD105-positive erythroid cells as compared with the CD34(+) progenitor compartment (2362 upregulated genes). A highly significant difference was observed in the expression level of genes involved in transcription, heme synthesis, iron and mitochondrial metabolism and transforming growth factor-β signaling. A comparison with recently published data showed over 1000 genes that as yet have not been reported to be upregulated in the early erythroid lineage. The gene expression level within distinct pathways could be illustrated directly by applying the Ingenuity software program. The results of gene expression analyses can be seen at the Gene Expression Omnibus repository. Nature Publishing Group 2013-01 2013-01-11 /pmc/articles/PMC3556575/ /pubmed/23310930 http://dx.doi.org/10.1038/bcj.2012.45 Text en Copyright © 2013 Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Machherndl-Spandl, S
Suessner, S
Danzer, M
Proell, J
Gabriel, C
Lauf, J
Sylie, R
Klein, H-U
Béné, M C
Weltermann, A
Bettelheim, P
Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title_full Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title_fullStr Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title_full_unstemmed Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title_short Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
title_sort molecular pathways of early cd105-positive erythroid cells as compared with cd34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556575/
https://www.ncbi.nlm.nih.gov/pubmed/23310930
http://dx.doi.org/10.1038/bcj.2012.45
work_keys_str_mv AT machherndlspandls molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT suessners molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT danzerm molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT proellj molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT gabrielc molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT laufj molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT sylier molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT kleinhu molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT benemc molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT weltermanna molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling
AT bettelheimp molecularpathwaysofearlycd105positiveerythroidcellsascomparedwithcd34positivecommonprecursorcellsbyflowcytometriccellsortingandgeneexpressionprofiling