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Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer

BACKGROUND: G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E(2)), tamoxifen, and ICI 182,780 (fulvestrant) and promotes activation of epidermal growth factor receptor (EGFR)-mediated signaling in breast, endometrial and thyroid cancer cells. Although lung adenoc...

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Autores principales: Jala, Venkatakrishna Rao, Radde, Brandie N, Haribabu, Bodduluri, Klinge, Carolyn M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557142/
https://www.ncbi.nlm.nih.gov/pubmed/23273253
http://dx.doi.org/10.1186/1471-2407-12-624
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author Jala, Venkatakrishna Rao
Radde, Brandie N
Haribabu, Bodduluri
Klinge, Carolyn M
author_facet Jala, Venkatakrishna Rao
Radde, Brandie N
Haribabu, Bodduluri
Klinge, Carolyn M
author_sort Jala, Venkatakrishna Rao
collection PubMed
description BACKGROUND: G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E(2)), tamoxifen, and ICI 182,780 (fulvestrant) and promotes activation of epidermal growth factor receptor (EGFR)-mediated signaling in breast, endometrial and thyroid cancer cells. Although lung adenocarcinomas express estrogen receptors α and β (ERα and ERβ), the expression of GPER in lung cancer has not been investigated. The purpose of this study was to examine the expression of GPER in lung cancer. METHODS: The expression patterns of GPER in various lung cancer lines and lung tumors were investigated using standard quantitative real time PCR (at mRNA levels), Western blot and immunohistochemistry (IHC) methods (at protein levels). The expression of GPER was scored and the pairwise comparisons (cancer vs adjacent tissues as well as cancer vs normal lung tissues) were performed. RESULTS: Analysis by real-time PCR and Western blotting revealed a significantly higher expression of GPER at both mRNA and protein levels in human non small cell lung cancer cell (NSCLC) lines relative to immortalized normal lung bronchial epithelial cells (HBECs). The virally immortalized human small airway epithelial cell line HPL1D showed higher expression than HBECs and similar expression to NSCLC cells. Immunohistochemical analysis of tissue sections of murine lung adenomas as well as human lung adenocarcinomas, squamous cell carcinomas and non-small cell lung carcinomas showed consistently higher expression of GPER in the tumor relative to the surrounding non-tumor tissue. CONCLUSION: The results from this study demonstrate increased GPER expression in lung cancer cells and tumors compared to normal lung. Further evaluation of the function and regulation of GPER will be necessary to determine if GPER is a marker of lung cancer progression.
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spelling pubmed-35571422013-01-31 Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer Jala, Venkatakrishna Rao Radde, Brandie N Haribabu, Bodduluri Klinge, Carolyn M BMC Cancer Research Article BACKGROUND: G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E(2)), tamoxifen, and ICI 182,780 (fulvestrant) and promotes activation of epidermal growth factor receptor (EGFR)-mediated signaling in breast, endometrial and thyroid cancer cells. Although lung adenocarcinomas express estrogen receptors α and β (ERα and ERβ), the expression of GPER in lung cancer has not been investigated. The purpose of this study was to examine the expression of GPER in lung cancer. METHODS: The expression patterns of GPER in various lung cancer lines and lung tumors were investigated using standard quantitative real time PCR (at mRNA levels), Western blot and immunohistochemistry (IHC) methods (at protein levels). The expression of GPER was scored and the pairwise comparisons (cancer vs adjacent tissues as well as cancer vs normal lung tissues) were performed. RESULTS: Analysis by real-time PCR and Western blotting revealed a significantly higher expression of GPER at both mRNA and protein levels in human non small cell lung cancer cell (NSCLC) lines relative to immortalized normal lung bronchial epithelial cells (HBECs). The virally immortalized human small airway epithelial cell line HPL1D showed higher expression than HBECs and similar expression to NSCLC cells. Immunohistochemical analysis of tissue sections of murine lung adenomas as well as human lung adenocarcinomas, squamous cell carcinomas and non-small cell lung carcinomas showed consistently higher expression of GPER in the tumor relative to the surrounding non-tumor tissue. CONCLUSION: The results from this study demonstrate increased GPER expression in lung cancer cells and tumors compared to normal lung. Further evaluation of the function and regulation of GPER will be necessary to determine if GPER is a marker of lung cancer progression. BioMed Central 2012-12-28 /pmc/articles/PMC3557142/ /pubmed/23273253 http://dx.doi.org/10.1186/1471-2407-12-624 Text en Copyright ©2012 Jala et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jala, Venkatakrishna Rao
Radde, Brandie N
Haribabu, Bodduluri
Klinge, Carolyn M
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title_full Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title_fullStr Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title_full_unstemmed Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title_short Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
title_sort enhanced expression of g-protein coupled estrogen receptor (gper/gpr30) in lung cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557142/
https://www.ncbi.nlm.nih.gov/pubmed/23273253
http://dx.doi.org/10.1186/1471-2407-12-624
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