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Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation
BACKGROUND: Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics in various cellular regions. In FLIP, a small cellular region is repeatedly illuminated by an intense laser pulse, while images are taken with reduced laser po...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557157/ https://www.ncbi.nlm.nih.gov/pubmed/23148417 http://dx.doi.org/10.1186/1471-2105-13-296 |