Cargando…
Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection
Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichom...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558142/ https://www.ncbi.nlm.nih.gov/pubmed/23408769 |
_version_ | 1782257379878895616 |
---|---|
author | Darani, Hossein Yousofi Ahmadi, Firuzeh Zabardast, Nozhat Yousefi, Hossein Ali Shirzad, Hedayat |
author_facet | Darani, Hossein Yousofi Ahmadi, Firuzeh Zabardast, Nozhat Yousefi, Hossein Ali Shirzad, Hedayat |
author_sort | Darani, Hossein Yousofi |
collection | PubMed |
description | Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex agglutination tests. Sensitivity and specificity of latex test was determined considering culture as golden standard. Sensitivity and specificity of latex agglutination test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex agglutination test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex agglutination test developed in this study, further work is recommended to improve the test. |
format | Online Article Text |
id | pubmed-3558142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-35581422013-02-13 Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection Darani, Hossein Yousofi Ahmadi, Firuzeh Zabardast, Nozhat Yousefi, Hossein Ali Shirzad, Hedayat Avicenna J Med Biotechnol Original Article Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex agglutination tests. Sensitivity and specificity of latex test was determined considering culture as golden standard. Sensitivity and specificity of latex agglutination test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex agglutination test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex agglutination test developed in this study, further work is recommended to improve the test. Avicenna Research Institute 2010 /pmc/articles/PMC3558142/ /pubmed/23408769 Text en Copyright © 2010 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Darani, Hossein Yousofi Ahmadi, Firuzeh Zabardast, Nozhat Yousefi, Hossein Ali Shirzad, Hedayat Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title | Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title_full | Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title_fullStr | Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title_full_unstemmed | Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title_short | Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection |
title_sort | development of a latex agglutination test as a simple and rapid method for diagnosis of trichomonas vaginalis infection |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558142/ https://www.ncbi.nlm.nih.gov/pubmed/23408769 |
work_keys_str_mv | AT daranihosseinyousofi developmentofalatexagglutinationtestasasimpleandrapidmethodfordiagnosisoftrichomonasvaginalisinfection AT ahmadifiruzeh developmentofalatexagglutinationtestasasimpleandrapidmethodfordiagnosisoftrichomonasvaginalisinfection AT zabardastnozhat developmentofalatexagglutinationtestasasimpleandrapidmethodfordiagnosisoftrichomonasvaginalisinfection AT yousefihosseinali developmentofalatexagglutinationtestasasimpleandrapidmethodfordiagnosisoftrichomonasvaginalisinfection AT shirzadhedayat developmentofalatexagglutinationtestasasimpleandrapidmethodfordiagnosisoftrichomonasvaginalisinfection |