Cargando…
The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice
Spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. The present study was designed to examine the effect of hCG treatment on germ cell proliferation following stem cell transplantation in mice. Spermatogonial stem cells were is...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558145/ https://www.ncbi.nlm.nih.gov/pubmed/23407454 |
_version_ | 1782257380576198656 |
---|---|
author | Akhondi, Mohammad Mehdi Najar, Reza Akbarzadeh Jeddi-Tehrani, Mahmood Sadeghi, Mohammad-Reza Zarnani, Amir Hassan Rabbani, Hodjattallah Salehkhou, Sheida Eini, Leila Hoseinzadeh, Fatemeh Heidari, Mahnaz |
author_facet | Akhondi, Mohammad Mehdi Najar, Reza Akbarzadeh Jeddi-Tehrani, Mahmood Sadeghi, Mohammad-Reza Zarnani, Amir Hassan Rabbani, Hodjattallah Salehkhou, Sheida Eini, Leila Hoseinzadeh, Fatemeh Heidari, Mahnaz |
author_sort | Akhondi, Mohammad Mehdi |
collection | PubMed |
description | Spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. The present study was designed to examine the effect of hCG treatment on germ cell proliferation following stem cell transplantation in mice. Spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and morphological analysis. hCG was injected into normal and cell transplanted mice. We then evaluated the testosterone levels and cell number in normal mice. After that, cyclin B1 gene expression was investigated in transplanted mice. Different doses of busulfan were injected to investigate the effects of chemotherapy on morphological criteria and preparation of recipient mice for transplantation. In this report we show proliferative potential of spermatogonial stem cells after cytotoxic treatment, transplantation efficiency by semi-quantitative RT-PCR, and hCG effect on stem cell regeneration in normal mice and following cell transplantation. The results indicate that spermatogonial stem cells can proliferate after transplantation, and the efficiency of their transplantation depends on hormonal treatment. Therefore, hormonal treatment after stem cell transplantation will be a powerful avenue for increasing the efficiency of transplantation and fertility restoration. |
format | Online Article Text |
id | pubmed-3558145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-35581452013-02-13 The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice Akhondi, Mohammad Mehdi Najar, Reza Akbarzadeh Jeddi-Tehrani, Mahmood Sadeghi, Mohammad-Reza Zarnani, Amir Hassan Rabbani, Hodjattallah Salehkhou, Sheida Eini, Leila Hoseinzadeh, Fatemeh Heidari, Mahnaz Avicenna J Med Biotechnol Original Article Spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. The present study was designed to examine the effect of hCG treatment on germ cell proliferation following stem cell transplantation in mice. Spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and morphological analysis. hCG was injected into normal and cell transplanted mice. We then evaluated the testosterone levels and cell number in normal mice. After that, cyclin B1 gene expression was investigated in transplanted mice. Different doses of busulfan were injected to investigate the effects of chemotherapy on morphological criteria and preparation of recipient mice for transplantation. In this report we show proliferative potential of spermatogonial stem cells after cytotoxic treatment, transplantation efficiency by semi-quantitative RT-PCR, and hCG effect on stem cell regeneration in normal mice and following cell transplantation. The results indicate that spermatogonial stem cells can proliferate after transplantation, and the efficiency of their transplantation depends on hormonal treatment. Therefore, hormonal treatment after stem cell transplantation will be a powerful avenue for increasing the efficiency of transplantation and fertility restoration. Avicenna Research Institute 2010 /pmc/articles/PMC3558145/ /pubmed/23407454 Text en Copyright © 2010 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Akhondi, Mohammad Mehdi Najar, Reza Akbarzadeh Jeddi-Tehrani, Mahmood Sadeghi, Mohammad-Reza Zarnani, Amir Hassan Rabbani, Hodjattallah Salehkhou, Sheida Eini, Leila Hoseinzadeh, Fatemeh Heidari, Mahnaz The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title | The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title_full | The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title_fullStr | The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title_full_unstemmed | The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title_short | The Effect of Human Chorionic Gonadotropin Treatment on Recipient Mouse Germ Cell Proliferation Following Spermatogonial Stem Cell Transplantation of Neonatal Donor Mice |
title_sort | effect of human chorionic gonadotropin treatment on recipient mouse germ cell proliferation following spermatogonial stem cell transplantation of neonatal donor mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558145/ https://www.ncbi.nlm.nih.gov/pubmed/23407454 |
work_keys_str_mv | AT akhondimohammadmehdi theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT najarrezaakbarzadeh theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT jedditehranimahmood theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT sadeghimohammadreza theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT zarnaniamirhassan theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT rabbanihodjattallah theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT salehkhousheida theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT einileila theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT hoseinzadehfatemeh theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT heidarimahnaz theeffectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT akhondimohammadmehdi effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT najarrezaakbarzadeh effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT jedditehranimahmood effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT sadeghimohammadreza effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT zarnaniamirhassan effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT rabbanihodjattallah effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT salehkhousheida effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT einileila effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT hoseinzadehfatemeh effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice AT heidarimahnaz effectofhumanchorionicgonadotropintreatmentonrecipientmousegermcellproliferationfollowingspermatogonialstemcelltransplantationofneonataldonormice |