Production of Monoclonal Antibody against Human Nestin

We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing h...

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Detalles Bibliográficos
Autores principales: Hadavi, Reza, Zarnani, Amir Hassan, Ahmadvand, Negah, Mahmoudi, Ahmad Reza, Bayat, Ali Ahmad, Mahmoudian, Jafar, Sadeghi, Mohammad-Reza, Soltanghoraee, Haleh, Akhondi, Mohammad Mehdi, Tarahomi, Majid, Jeddi-Tehrani, Mahmood, Rabbani, Hodjattallah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2010
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558152/
https://www.ncbi.nlm.nih.gov/pubmed/23407796
Descripción
Sumario:We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140–250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.

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