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Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts

Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cel...

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Autores principales: Qasemi-Panahi, Babak, Tajik, Parviz, Movahedin, Mansoureh, Moghaddam, Gholamali, Barzgar, Younes, Heidari-Vala, Hamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558187/
https://www.ncbi.nlm.nih.gov/pubmed/23408761
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author Qasemi-Panahi, Babak
Tajik, Parviz
Movahedin, Mansoureh
Moghaddam, Gholamali
Barzgar, Younes
Heidari-Vala, Hamed
author_facet Qasemi-Panahi, Babak
Tajik, Parviz
Movahedin, Mansoureh
Moghaddam, Gholamali
Barzgar, Younes
Heidari-Vala, Hamed
author_sort Qasemi-Panahi, Babak
collection PubMed
description Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining.
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spelling pubmed-35581872013-02-13 Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts Qasemi-Panahi, Babak Tajik, Parviz Movahedin, Mansoureh Moghaddam, Gholamali Barzgar, Younes Heidari-Vala, Hamed Avicenna J Med Biotechnol Original Article Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining. Avicenna Research Institute 2011 /pmc/articles/PMC3558187/ /pubmed/23408761 Text en Copyright © 2011 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Qasemi-Panahi, Babak
Tajik, Parviz
Movahedin, Mansoureh
Moghaddam, Gholamali
Barzgar, Younes
Heidari-Vala, Hamed
Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title_full Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title_fullStr Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title_full_unstemmed Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title_short Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
title_sort differentiation of bovine spermatogonial stem cells into osteoblasts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558187/
https://www.ncbi.nlm.nih.gov/pubmed/23408761
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