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Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation
In previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. Low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Avicenna Research Institute
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558193/ https://www.ncbi.nlm.nih.gov/pubmed/23407464 |
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author | Zargari, Maryam Sadeghi, Mohammad Reza Shahhosseiny, Mohammad Hassan Kamali, Koroush Saliminejad, Kyomars Esmaeilzadeh, Ali Khorshid, Hamid Reza Khorram |
author_facet | Zargari, Maryam Sadeghi, Mohammad Reza Shahhosseiny, Mohammad Hassan Kamali, Koroush Saliminejad, Kyomars Esmaeilzadeh, Ali Khorshid, Hamid Reza Khorram |
author_sort | Zargari, Maryam |
collection | PubMed |
description | In previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. Low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. With the discovery of cell-free fetal DNA (cffDNA) in plasma of pregnant women, access to genetic material of the fetus had become possible to determine early gender of a fetus in pregnancies at the risk of X-linked genetic conditions instead of applying invasive methods. Therefore in this study, the probability of detecting sequences on the Y chromosome in pregnant women has been evaluated to identify the gender of fetuses. Peripheral blood samples were obtained from 80 pregnant women at 6(th) to 10(th) weeks of gestation and then the fetal DNA was extracted from the plasma. Nested PCR was applied to detect the sequences of single copy SRY gene and multi copy DYS14 & DAZ genes on the Y chromosome of the male fetuses. At the end, all the obtained results were compared with the actual gender of the newborns. In 40 out of 42 born baby boys, the relevant gene sequences were identified and 95.2% sensitivity was obtained. Non-invasive early determination of fetal gender using cffDNA could be employed as a pre-test in the shortest possible time and with a high reliability to avoid applying invasive methods in cases where a fetus is at the risk of genetic diseases. |
format | Online Article Text |
id | pubmed-3558193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-35581932013-02-13 Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation Zargari, Maryam Sadeghi, Mohammad Reza Shahhosseiny, Mohammad Hassan Kamali, Koroush Saliminejad, Kyomars Esmaeilzadeh, Ali Khorshid, Hamid Reza Khorram Avicenna J Med Biotechnol Original Article In previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. Low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. With the discovery of cell-free fetal DNA (cffDNA) in plasma of pregnant women, access to genetic material of the fetus had become possible to determine early gender of a fetus in pregnancies at the risk of X-linked genetic conditions instead of applying invasive methods. Therefore in this study, the probability of detecting sequences on the Y chromosome in pregnant women has been evaluated to identify the gender of fetuses. Peripheral blood samples were obtained from 80 pregnant women at 6(th) to 10(th) weeks of gestation and then the fetal DNA was extracted from the plasma. Nested PCR was applied to detect the sequences of single copy SRY gene and multi copy DYS14 & DAZ genes on the Y chromosome of the male fetuses. At the end, all the obtained results were compared with the actual gender of the newborns. In 40 out of 42 born baby boys, the relevant gene sequences were identified and 95.2% sensitivity was obtained. Non-invasive early determination of fetal gender using cffDNA could be employed as a pre-test in the shortest possible time and with a high reliability to avoid applying invasive methods in cases where a fetus is at the risk of genetic diseases. Avicenna Research Institute 2011 /pmc/articles/PMC3558193/ /pubmed/23407464 Text en Copyright © 2011 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Zargari, Maryam Sadeghi, Mohammad Reza Shahhosseiny, Mohammad Hassan Kamali, Koroush Saliminejad, Kyomars Esmaeilzadeh, Ali Khorshid, Hamid Reza Khorram Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title | Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title_full | Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title_fullStr | Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title_full_unstemmed | Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title_short | Fetal Sex Determination using Non-Invasive Method of Cell-free Fetal DNA in Maternal Plasma of Pregnant Women During 6(th)– 10(th) Weeks of Gestation |
title_sort | fetal sex determination using non-invasive method of cell-free fetal dna in maternal plasma of pregnant women during 6(th)– 10(th) weeks of gestation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558193/ https://www.ncbi.nlm.nih.gov/pubmed/23407464 |
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