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3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines

BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim...

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Autores principales: Bokharaei, Hanieh, Raz, Abbasali, Zakeri, Sedigheh, Djadid, Navid Dinparast
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558215/
https://www.ncbi.nlm.nih.gov/pubmed/23407363
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author Bokharaei, Hanieh
Raz, Abbasali
Zakeri, Sedigheh
Djadid, Navid Dinparast
author_facet Bokharaei, Hanieh
Raz, Abbasali
Zakeri, Sedigheh
Djadid, Navid Dinparast
author_sort Bokharaei, Hanieh
collection PubMed
description BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim at interfering and/or blocking pathogen development within the vector, halting transmission to non-infected vertebrate host. Aminopeptidase N (APN) is one of the most potent proteins in parasite development in Anopheles malaria vectors, which is strongly co-localized with human malaria parasites in the mosquito midgut epithelium. Therefore, Aminopeptidase N is one of the best choices for a new TBV. METHODS: In this study for the first time we used 3’-RACE to amplify APN gene in Anopheles stephensi (An.stephensi), a major malaria vector in Iran, Indian subcontinent up to China by using different sets of primers including exon junction, conserved and specific region primers. RESULTS: Full length of APN was sequenced stepwise, which could be applied in designing a new regional TBV and act as an essential component of malaria elimination program in An.stephensi distribution areas. CONCLUSION: Primers design and method modification should be set up exactly in approach based amplifications. From results we came to this conclusion that that 3’-RACE could be applied to amplified key regions which are beyond reach.
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spelling pubmed-35582152013-02-13 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines Bokharaei, Hanieh Raz, Abbasali Zakeri, Sedigheh Djadid, Navid Dinparast Avicenna J Med Biotechnol Original Article BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim at interfering and/or blocking pathogen development within the vector, halting transmission to non-infected vertebrate host. Aminopeptidase N (APN) is one of the most potent proteins in parasite development in Anopheles malaria vectors, which is strongly co-localized with human malaria parasites in the mosquito midgut epithelium. Therefore, Aminopeptidase N is one of the best choices for a new TBV. METHODS: In this study for the first time we used 3’-RACE to amplify APN gene in Anopheles stephensi (An.stephensi), a major malaria vector in Iran, Indian subcontinent up to China by using different sets of primers including exon junction, conserved and specific region primers. RESULTS: Full length of APN was sequenced stepwise, which could be applied in designing a new regional TBV and act as an essential component of malaria elimination program in An.stephensi distribution areas. CONCLUSION: Primers design and method modification should be set up exactly in approach based amplifications. From results we came to this conclusion that that 3’-RACE could be applied to amplified key regions which are beyond reach. Avicenna Research Institute 2012 /pmc/articles/PMC3558215/ /pubmed/23407363 Text en Copyright © 2012 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Bokharaei, Hanieh
Raz, Abbasali
Zakeri, Sedigheh
Djadid, Navid Dinparast
3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title_full 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title_fullStr 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title_full_unstemmed 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title_short 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
title_sort 3’-race amplification of aminopeptidase n gene from anopheles stephensi applicable in transmission blocking vaccines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558215/
https://www.ncbi.nlm.nih.gov/pubmed/23407363
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