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3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines
BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558215/ https://www.ncbi.nlm.nih.gov/pubmed/23407363 |
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author | Bokharaei, Hanieh Raz, Abbasali Zakeri, Sedigheh Djadid, Navid Dinparast |
author_facet | Bokharaei, Hanieh Raz, Abbasali Zakeri, Sedigheh Djadid, Navid Dinparast |
author_sort | Bokharaei, Hanieh |
collection | PubMed |
description | BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim at interfering and/or blocking pathogen development within the vector, halting transmission to non-infected vertebrate host. Aminopeptidase N (APN) is one of the most potent proteins in parasite development in Anopheles malaria vectors, which is strongly co-localized with human malaria parasites in the mosquito midgut epithelium. Therefore, Aminopeptidase N is one of the best choices for a new TBV. METHODS: In this study for the first time we used 3’-RACE to amplify APN gene in Anopheles stephensi (An.stephensi), a major malaria vector in Iran, Indian subcontinent up to China by using different sets of primers including exon junction, conserved and specific region primers. RESULTS: Full length of APN was sequenced stepwise, which could be applied in designing a new regional TBV and act as an essential component of malaria elimination program in An.stephensi distribution areas. CONCLUSION: Primers design and method modification should be set up exactly in approach based amplifications. From results we came to this conclusion that that 3’-RACE could be applied to amplified key regions which are beyond reach. |
format | Online Article Text |
id | pubmed-3558215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-35582152013-02-13 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines Bokharaei, Hanieh Raz, Abbasali Zakeri, Sedigheh Djadid, Navid Dinparast Avicenna J Med Biotechnol Original Article BACKGROUND: Because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) Transmission-Blocking Vaccines (TBVs) concept has been raised in recent years, promising a more efficient way to malaria control. TBVs aim at interfering and/or blocking pathogen development within the vector, halting transmission to non-infected vertebrate host. Aminopeptidase N (APN) is one of the most potent proteins in parasite development in Anopheles malaria vectors, which is strongly co-localized with human malaria parasites in the mosquito midgut epithelium. Therefore, Aminopeptidase N is one of the best choices for a new TBV. METHODS: In this study for the first time we used 3’-RACE to amplify APN gene in Anopheles stephensi (An.stephensi), a major malaria vector in Iran, Indian subcontinent up to China by using different sets of primers including exon junction, conserved and specific region primers. RESULTS: Full length of APN was sequenced stepwise, which could be applied in designing a new regional TBV and act as an essential component of malaria elimination program in An.stephensi distribution areas. CONCLUSION: Primers design and method modification should be set up exactly in approach based amplifications. From results we came to this conclusion that that 3’-RACE could be applied to amplified key regions which are beyond reach. Avicenna Research Institute 2012 /pmc/articles/PMC3558215/ /pubmed/23407363 Text en Copyright © 2012 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Bokharaei, Hanieh Raz, Abbasali Zakeri, Sedigheh Djadid, Navid Dinparast 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title | 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title_full | 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title_fullStr | 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title_full_unstemmed | 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title_short | 3’-RACE Amplification of Aminopeptidase N Gene from Anopheles stephensi Applicable in Transmission Blocking Vaccines |
title_sort | 3’-race amplification of aminopeptidase n gene from anopheles stephensi applicable in transmission blocking vaccines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558215/ https://www.ncbi.nlm.nih.gov/pubmed/23407363 |
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