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The genetic regulation of the terminating phase of liver regeneration
BACKGROUND: After partial hepatectomy (PHx), the liver regeneration process terminates when the normal liver-mass/body-weight ratio of 2.5% has been re-established. To investigate the genetic regulation of the terminating phase of liver regeneration, we performed a 60% PHx in a porcine model. Liver...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558440/ https://www.ncbi.nlm.nih.gov/pubmed/23164283 http://dx.doi.org/10.1186/1476-5926-11-3 |
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author | Nygård, Ingvild E Mortensen, Kim E Hedegaard, Jakob Conley, Lene N Kalstad, Trine Bendixen, Christian Revhaug, Arthur |
author_facet | Nygård, Ingvild E Mortensen, Kim E Hedegaard, Jakob Conley, Lene N Kalstad, Trine Bendixen, Christian Revhaug, Arthur |
author_sort | Nygård, Ingvild E |
collection | PubMed |
description | BACKGROUND: After partial hepatectomy (PHx), the liver regeneration process terminates when the normal liver-mass/body-weight ratio of 2.5% has been re-established. To investigate the genetic regulation of the terminating phase of liver regeneration, we performed a 60% PHx in a porcine model. Liver biopsies were taken at the time of resection, after three weeks and upon termination the sixth week. Gene expression profiles were obtained using porcine oligonucleotide microarrays. Our study reveals the interactions between genes regulating the cell cycle, apoptosis and angiogenesis, and the role of Transforming Growth Factor-β (TGF-β) signalling towards the end of liver regeneration. RESULTS: Microarray analysis revealed a dominance of genes regulating apoptosis towards the end of regeneration. Caspase Recruitment Domain-Containing Protein 11 (CARD11) was up-regulated six weeks after PHx, suggesting the involvement of the caspase system at this time. Zinc Finger Protein (ZNF490) gene, with a potential negative effect on cell cycle progression, was only up-regulated at three and six weeks after PHx indicating a central role at this time. TGF-β regulation was not found to be significantly affected in the terminating phase of liver regeneration. Vasohibin 2 (VASH2) was down-regulated towards the end of regeneration, and may indicate a role in preventing a continued vascularization process. CONCLUSIONS: CARD11, ZNF490 and VASH2 are differentially expressed in the termination phase of liver regeneration. The lack of TGF-β up-regulation suggests that signalling by TGF-β is not required for termination of liver regeneration. |
format | Online Article Text |
id | pubmed-3558440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35584402013-01-31 The genetic regulation of the terminating phase of liver regeneration Nygård, Ingvild E Mortensen, Kim E Hedegaard, Jakob Conley, Lene N Kalstad, Trine Bendixen, Christian Revhaug, Arthur Comp Hepatol Research BACKGROUND: After partial hepatectomy (PHx), the liver regeneration process terminates when the normal liver-mass/body-weight ratio of 2.5% has been re-established. To investigate the genetic regulation of the terminating phase of liver regeneration, we performed a 60% PHx in a porcine model. Liver biopsies were taken at the time of resection, after three weeks and upon termination the sixth week. Gene expression profiles were obtained using porcine oligonucleotide microarrays. Our study reveals the interactions between genes regulating the cell cycle, apoptosis and angiogenesis, and the role of Transforming Growth Factor-β (TGF-β) signalling towards the end of liver regeneration. RESULTS: Microarray analysis revealed a dominance of genes regulating apoptosis towards the end of regeneration. Caspase Recruitment Domain-Containing Protein 11 (CARD11) was up-regulated six weeks after PHx, suggesting the involvement of the caspase system at this time. Zinc Finger Protein (ZNF490) gene, with a potential negative effect on cell cycle progression, was only up-regulated at three and six weeks after PHx indicating a central role at this time. TGF-β regulation was not found to be significantly affected in the terminating phase of liver regeneration. Vasohibin 2 (VASH2) was down-regulated towards the end of regeneration, and may indicate a role in preventing a continued vascularization process. CONCLUSIONS: CARD11, ZNF490 and VASH2 are differentially expressed in the termination phase of liver regeneration. The lack of TGF-β up-regulation suggests that signalling by TGF-β is not required for termination of liver regeneration. BioMed Central 2012-11-20 /pmc/articles/PMC3558440/ /pubmed/23164283 http://dx.doi.org/10.1186/1476-5926-11-3 Text en Copyright ©2012 Nygård et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Nygård, Ingvild E Mortensen, Kim E Hedegaard, Jakob Conley, Lene N Kalstad, Trine Bendixen, Christian Revhaug, Arthur The genetic regulation of the terminating phase of liver regeneration |
title | The genetic regulation of the terminating phase of liver regeneration |
title_full | The genetic regulation of the terminating phase of liver regeneration |
title_fullStr | The genetic regulation of the terminating phase of liver regeneration |
title_full_unstemmed | The genetic regulation of the terminating phase of liver regeneration |
title_short | The genetic regulation of the terminating phase of liver regeneration |
title_sort | genetic regulation of the terminating phase of liver regeneration |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558440/ https://www.ncbi.nlm.nih.gov/pubmed/23164283 http://dx.doi.org/10.1186/1476-5926-11-3 |
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