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A simple medium enables bovine embryos to be held for seven days at 4°C
Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of tra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558692/ https://www.ncbi.nlm.nih.gov/pubmed/23378907 http://dx.doi.org/10.1038/srep01173 |
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author | Ideta, Atsushi Aoyagi, Yoshito Tsuchiya, Kanami Kamijima, Tatsuro Nishimiya, Yoshiyuki Tsuda, Sakae |
author_facet | Ideta, Atsushi Aoyagi, Yoshito Tsuchiya, Kanami Kamijima, Tatsuro Nishimiya, Yoshiyuki Tsuda, Sakae |
author_sort | Ideta, Atsushi |
collection | PubMed |
description | Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days. |
format | Online Article Text |
id | pubmed-3558692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35586922013-02-01 A simple medium enables bovine embryos to be held for seven days at 4°C Ideta, Atsushi Aoyagi, Yoshito Tsuchiya, Kanami Kamijima, Tatsuro Nishimiya, Yoshiyuki Tsuda, Sakae Sci Rep Article Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days. Nature Publishing Group 2013-01-30 /pmc/articles/PMC3558692/ /pubmed/23378907 http://dx.doi.org/10.1038/srep01173 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Ideta, Atsushi Aoyagi, Yoshito Tsuchiya, Kanami Kamijima, Tatsuro Nishimiya, Yoshiyuki Tsuda, Sakae A simple medium enables bovine embryos to be held for seven days at 4°C |
title | A simple medium enables bovine embryos to be held for seven days at 4°C |
title_full | A simple medium enables bovine embryos to be held for seven days at 4°C |
title_fullStr | A simple medium enables bovine embryos to be held for seven days at 4°C |
title_full_unstemmed | A simple medium enables bovine embryos to be held for seven days at 4°C |
title_short | A simple medium enables bovine embryos to be held for seven days at 4°C |
title_sort | simple medium enables bovine embryos to be held for seven days at 4°c |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558692/ https://www.ncbi.nlm.nih.gov/pubmed/23378907 http://dx.doi.org/10.1038/srep01173 |
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