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A simple medium enables bovine embryos to be held for seven days at 4°C

Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of tra...

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Autores principales: Ideta, Atsushi, Aoyagi, Yoshito, Tsuchiya, Kanami, Kamijima, Tatsuro, Nishimiya, Yoshiyuki, Tsuda, Sakae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558692/
https://www.ncbi.nlm.nih.gov/pubmed/23378907
http://dx.doi.org/10.1038/srep01173
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author Ideta, Atsushi
Aoyagi, Yoshito
Tsuchiya, Kanami
Kamijima, Tatsuro
Nishimiya, Yoshiyuki
Tsuda, Sakae
author_facet Ideta, Atsushi
Aoyagi, Yoshito
Tsuchiya, Kanami
Kamijima, Tatsuro
Nishimiya, Yoshiyuki
Tsuda, Sakae
author_sort Ideta, Atsushi
collection PubMed
description Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days.
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spelling pubmed-35586922013-02-01 A simple medium enables bovine embryos to be held for seven days at 4°C Ideta, Atsushi Aoyagi, Yoshito Tsuchiya, Kanami Kamijima, Tatsuro Nishimiya, Yoshiyuki Tsuda, Sakae Sci Rep Article Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days. Nature Publishing Group 2013-01-30 /pmc/articles/PMC3558692/ /pubmed/23378907 http://dx.doi.org/10.1038/srep01173 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Ideta, Atsushi
Aoyagi, Yoshito
Tsuchiya, Kanami
Kamijima, Tatsuro
Nishimiya, Yoshiyuki
Tsuda, Sakae
A simple medium enables bovine embryos to be held for seven days at 4°C
title A simple medium enables bovine embryos to be held for seven days at 4°C
title_full A simple medium enables bovine embryos to be held for seven days at 4°C
title_fullStr A simple medium enables bovine embryos to be held for seven days at 4°C
title_full_unstemmed A simple medium enables bovine embryos to be held for seven days at 4°C
title_short A simple medium enables bovine embryos to be held for seven days at 4°C
title_sort simple medium enables bovine embryos to be held for seven days at 4°c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558692/
https://www.ncbi.nlm.nih.gov/pubmed/23378907
http://dx.doi.org/10.1038/srep01173
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