Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli

Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia c...

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Autores principales: Traglia, German M., Sala, Carol Davies, Fuxman Bass, Juan I., Soler-Bistué, Alfonso J.C., Zorreguieta, Angeles, Ramírez, María Soledad, Tolmasky, Marcelo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2012
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559211/
https://www.ncbi.nlm.nih.gov/pubmed/23515318
http://dx.doi.org/10.1089/biores.2012.0257
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author Traglia, German M.
Sala, Carol Davies
Fuxman Bass, Juan I.
Soler-Bistué, Alfonso J.C.
Zorreguieta, Angeles
Ramírez, María Soledad
Tolmasky, Marcelo E.
author_facet Traglia, German M.
Sala, Carol Davies
Fuxman Bass, Juan I.
Soler-Bistué, Alfonso J.C.
Zorreguieta, Angeles
Ramírez, María Soledad
Tolmasky, Marcelo E.
author_sort Traglia, German M.
collection PubMed
description Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.
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spelling pubmed-35592112013-03-20 Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli Traglia, German M. Sala, Carol Davies Fuxman Bass, Juan I. Soler-Bistué, Alfonso J.C. Zorreguieta, Angeles Ramírez, María Soledad Tolmasky, Marcelo E. Biores Open Access Brief Reports Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm. Mary Ann Liebert, Inc. 2012-10 /pmc/articles/PMC3559211/ /pubmed/23515318 http://dx.doi.org/10.1089/biores.2012.0257 Text en Copyright 2012, Mary Ann Liebert, Inc.
spellingShingle Brief Reports
Traglia, German M.
Sala, Carol Davies
Fuxman Bass, Juan I.
Soler-Bistué, Alfonso J.C.
Zorreguieta, Angeles
Ramírez, María Soledad
Tolmasky, Marcelo E.
Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title_full Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title_fullStr Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title_full_unstemmed Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title_short Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
title_sort internalization of locked nucleic acids/dna hybrid oligomers into escherichia coli
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559211/
https://www.ncbi.nlm.nih.gov/pubmed/23515318
http://dx.doi.org/10.1089/biores.2012.0257
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