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A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold

We present a laminar flow reactor for bone tissue engineering that was developed based on a computational fluid dynamics model. The bioreactor design permits a laminar flow field through its specific internal shape. An integrated bypass system that prevents pressure build-up through bypass openings...

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Autores principales: Weyand, Birgit, Kasper, Cornelia, Israelowitz, Meir, Gille, Christoph, von Schroeder, Herbert P., Reimers, Kerstin, Vogt, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559213/
https://www.ncbi.nlm.nih.gov/pubmed/23515420
http://dx.doi.org/10.1089/biores.2012.9901
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author Weyand, Birgit
Kasper, Cornelia
Israelowitz, Meir
Gille, Christoph
von Schroeder, Herbert P.
Reimers, Kerstin
Vogt, Peter M.
author_facet Weyand, Birgit
Kasper, Cornelia
Israelowitz, Meir
Gille, Christoph
von Schroeder, Herbert P.
Reimers, Kerstin
Vogt, Peter M.
author_sort Weyand, Birgit
collection PubMed
description We present a laminar flow reactor for bone tissue engineering that was developed based on a computational fluid dynamics model. The bioreactor design permits a laminar flow field through its specific internal shape. An integrated bypass system that prevents pressure build-up through bypass openings for pressure release allows for a constant pressure environment during the changing of permeability values that are caused by cellular growth within a porous scaffold. A macroporous ceramic scaffold, composed of zirconium dioxide, was used as a test biomaterial that studies adipose stem cell behavior within a controlled three-dimensional (3D) flow and pressure environment. The topographic structure of the material provided a basis for stem cell proliferation and differentiation toward the osteogenic lineage. Dynamic culture conditions in the bioreactor supported cell viability during long-term culture and induced cell cluster formation and extra-cellular matrix deposition within the porous scaffold, though no complete closure of the pores with new-formed tissue was observed. We postulate that our system is suitable for studying fluid shear stress effects on stem cell proliferation and differentiation toward bone formation in tissue-engineered 3D constructs.
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spelling pubmed-35592132013-03-20 A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold Weyand, Birgit Kasper, Cornelia Israelowitz, Meir Gille, Christoph von Schroeder, Herbert P. Reimers, Kerstin Vogt, Peter M. Biores Open Access Article We present a laminar flow reactor for bone tissue engineering that was developed based on a computational fluid dynamics model. The bioreactor design permits a laminar flow field through its specific internal shape. An integrated bypass system that prevents pressure build-up through bypass openings for pressure release allows for a constant pressure environment during the changing of permeability values that are caused by cellular growth within a porous scaffold. A macroporous ceramic scaffold, composed of zirconium dioxide, was used as a test biomaterial that studies adipose stem cell behavior within a controlled three-dimensional (3D) flow and pressure environment. The topographic structure of the material provided a basis for stem cell proliferation and differentiation toward the osteogenic lineage. Dynamic culture conditions in the bioreactor supported cell viability during long-term culture and induced cell cluster formation and extra-cellular matrix deposition within the porous scaffold, though no complete closure of the pores with new-formed tissue was observed. We postulate that our system is suitable for studying fluid shear stress effects on stem cell proliferation and differentiation toward bone formation in tissue-engineered 3D constructs. Mary Ann Liebert, Inc. 2012-06 /pmc/articles/PMC3559213/ /pubmed/23515420 http://dx.doi.org/10.1089/biores.2012.9901 Text en Copyright 2012, Mary Ann Liebert, Inc.
spellingShingle Article
Weyand, Birgit
Kasper, Cornelia
Israelowitz, Meir
Gille, Christoph
von Schroeder, Herbert P.
Reimers, Kerstin
Vogt, Peter M.
A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title_full A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title_fullStr A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title_full_unstemmed A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title_short A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold
title_sort differential pressure laminar flow reactor supports osteogenic differentiation and extracellular matrix formation from adipose mesenchymal stem cells in a macroporous ceramic scaffold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559213/
https://www.ncbi.nlm.nih.gov/pubmed/23515420
http://dx.doi.org/10.1089/biores.2012.9901
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