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Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β
In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-ba...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559830/ https://www.ncbi.nlm.nih.gov/pubmed/23382991 http://dx.doi.org/10.1371/journal.pone.0054862 |
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author | Hirai, Hideyo Kamio, Naoka Huang, Gang Matsusue, Akiko Ogino, Shinpei Kimura, Nobuhiko Satake, Sakiko Ashihara, Eishi Imanishi, Jiro Tenen, Daniel G. Maekawa, Taira |
author_facet | Hirai, Hideyo Kamio, Naoka Huang, Gang Matsusue, Akiko Ogino, Shinpei Kimura, Nobuhiko Satake, Sakiko Ashihara, Eishi Imanishi, Jiro Tenen, Daniel G. Maekawa, Taira |
author_sort | Hirai, Hideyo |
collection | PubMed |
description | In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)–induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. |
format | Online Article Text |
id | pubmed-3559830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35598302013-02-04 Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β Hirai, Hideyo Kamio, Naoka Huang, Gang Matsusue, Akiko Ogino, Shinpei Kimura, Nobuhiko Satake, Sakiko Ashihara, Eishi Imanishi, Jiro Tenen, Daniel G. Maekawa, Taira PLoS One Research Article In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)–induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. Public Library of Science 2013-01-30 /pmc/articles/PMC3559830/ /pubmed/23382991 http://dx.doi.org/10.1371/journal.pone.0054862 Text en © 2013 Hirai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hirai, Hideyo Kamio, Naoka Huang, Gang Matsusue, Akiko Ogino, Shinpei Kimura, Nobuhiko Satake, Sakiko Ashihara, Eishi Imanishi, Jiro Tenen, Daniel G. Maekawa, Taira Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title | Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title_full | Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title_fullStr | Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title_full_unstemmed | Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title_short | Cyclic AMP Responsive Element Binding Proteins Are Involved in ‘Emergency’ Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β |
title_sort | cyclic amp responsive element binding proteins are involved in ‘emergency’ granulopoiesis through the upregulation of ccaat/enhancer binding protein β |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559830/ https://www.ncbi.nlm.nih.gov/pubmed/23382991 http://dx.doi.org/10.1371/journal.pone.0054862 |
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