Cargando…
Culture models to study leukocyte trafficking across the choroid plexus
BACKGROUND: A critical point during the course of central nervous system infection is the influx of leukocytes from the blood into the brain across the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). However, experimental in vitro models to investigate leukocyte transmig...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560101/ https://www.ncbi.nlm.nih.gov/pubmed/23305147 http://dx.doi.org/10.1186/2045-8118-10-1 |
_version_ | 1782257731916267520 |
---|---|
author | Tenenbaum, Tobias Steinmann, Ulrike Friedrich, Corinna Berger, Jürgen Schwerk, Christian Schroten, Horst |
author_facet | Tenenbaum, Tobias Steinmann, Ulrike Friedrich, Corinna Berger, Jürgen Schwerk, Christian Schroten, Horst |
author_sort | Tenenbaum, Tobias |
collection | PubMed |
description | BACKGROUND: A critical point during the course of central nervous system infection is the influx of leukocytes from the blood into the brain across the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). However, experimental in vitro models to investigate leukocyte transmigration across cultured choroid plexus epithelial cells have been lacking so far. METHODS: We have developed a porcine and human “inverted” culture insert system that enables leukocyte transmigration specifically from the physiologically relevant basolateral side. The models use primary porcine choroid plexus epithelial cells (PCPEC) and human choroid plexus papilloma cells (HIBCPP). As a prerequisite for a functional barrier, we optimized culture conditions in which cells are maintained in serum-containing medium until high barrier function is reached. Leukocyte transmigration through the plexus epithelial cells is analysed by three-dimensional Apotome(®)-imaging and electron microscopy, and the route of transmigration through the plexus epithelial cells, i.e. transcellular as well as paracellular, can be determined. DISCUSSION: As a functionally relevant porcine and human BCSFB model, PCPEC and HIBCPP respectively, offer a wide range of options for analysis of disease-related mechanisms at the choroid plexus epithelium, especially involving human pathogens. Moreover, our in vitro models facilitate the investigation of leukocyte entry into the CNS via the blood-CSF barrier. |
format | Online Article Text |
id | pubmed-3560101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35601012013-02-04 Culture models to study leukocyte trafficking across the choroid plexus Tenenbaum, Tobias Steinmann, Ulrike Friedrich, Corinna Berger, Jürgen Schwerk, Christian Schroten, Horst Fluids Barriers CNS Study Protocol BACKGROUND: A critical point during the course of central nervous system infection is the influx of leukocytes from the blood into the brain across the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). However, experimental in vitro models to investigate leukocyte transmigration across cultured choroid plexus epithelial cells have been lacking so far. METHODS: We have developed a porcine and human “inverted” culture insert system that enables leukocyte transmigration specifically from the physiologically relevant basolateral side. The models use primary porcine choroid plexus epithelial cells (PCPEC) and human choroid plexus papilloma cells (HIBCPP). As a prerequisite for a functional barrier, we optimized culture conditions in which cells are maintained in serum-containing medium until high barrier function is reached. Leukocyte transmigration through the plexus epithelial cells is analysed by three-dimensional Apotome(®)-imaging and electron microscopy, and the route of transmigration through the plexus epithelial cells, i.e. transcellular as well as paracellular, can be determined. DISCUSSION: As a functionally relevant porcine and human BCSFB model, PCPEC and HIBCPP respectively, offer a wide range of options for analysis of disease-related mechanisms at the choroid plexus epithelium, especially involving human pathogens. Moreover, our in vitro models facilitate the investigation of leukocyte entry into the CNS via the blood-CSF barrier. BioMed Central 2013-01-10 /pmc/articles/PMC3560101/ /pubmed/23305147 http://dx.doi.org/10.1186/2045-8118-10-1 Text en Copyright ©2013 Tenenbaum et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Study Protocol Tenenbaum, Tobias Steinmann, Ulrike Friedrich, Corinna Berger, Jürgen Schwerk, Christian Schroten, Horst Culture models to study leukocyte trafficking across the choroid plexus |
title | Culture models to study leukocyte trafficking across the choroid plexus |
title_full | Culture models to study leukocyte trafficking across the choroid plexus |
title_fullStr | Culture models to study leukocyte trafficking across the choroid plexus |
title_full_unstemmed | Culture models to study leukocyte trafficking across the choroid plexus |
title_short | Culture models to study leukocyte trafficking across the choroid plexus |
title_sort | culture models to study leukocyte trafficking across the choroid plexus |
topic | Study Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560101/ https://www.ncbi.nlm.nih.gov/pubmed/23305147 http://dx.doi.org/10.1186/2045-8118-10-1 |
work_keys_str_mv | AT tenenbaumtobias culturemodelstostudyleukocytetraffickingacrossthechoroidplexus AT steinmannulrike culturemodelstostudyleukocytetraffickingacrossthechoroidplexus AT friedrichcorinna culturemodelstostudyleukocytetraffickingacrossthechoroidplexus AT bergerjurgen culturemodelstostudyleukocytetraffickingacrossthechoroidplexus AT schwerkchristian culturemodelstostudyleukocytetraffickingacrossthechoroidplexus AT schrotenhorst culturemodelstostudyleukocytetraffickingacrossthechoroidplexus |